Muuss Marcel, Karaivanova Margarita, Skrzypczyk Lea, Wohlfart Sabrina, Herth Jonathan, Uhl Philipp, Auffarth Gerd Uwe, Hammer Maximilian
Heidelberg University Hospital, Department of Ophthalmology, Heidelberg, Germany.
David J. Apple Laboratory for Vision Research, Heidelberg, Germany.
Invest Ophthalmol Vis Sci. 2025 Aug 1;66(11):21. doi: 10.1167/iovs.66.11.21.
The purpose of this study was to investigate the effect of emulsified silicone oil on trabecular outflow resistance using an in vitro perfusion model of human and porcine anterior segments. F4H5, an approved washout for emulsified silicone oil in the posterior segment, was evaluated as a potential rescue treatment.
Seventeen porcine and 15 human anterior segments were dissected and clamped into an in vitro perfusion model. The intraocular pressure (IOP) was recorded continuously and evaluated before and after the injection of emulsified silicone oil, followed by treatment with F4H5 in the intervention group. Control eyes received injections of balanced salt solution (BSS).
In human eyes, the injection of emulsified silicone oil significantly increased IOP from 7.8 ± 1.3 millimeters of mercury (mm Hg) to 15.8 ± 3.5 mm Hg (increase of 8.0 ± 2.3 mm Hg, P = 0.02, mean time to stabilized higher pressure = 8.6 hours). Subsequent treatment with F4H5 reverted the IOP back to near baseline values (10.8 ± 2.2 mm Hg, P = 0.06 compared to baseline, mean time to stabilized lower pressure = 6.9 hours). Porcine eyes demonstrated comparable trends, with a significant IOP increase from 14.5 ± 1.2 mm Hg to 17.7 ± 1.6 mm Hg after silicone oil injection (increase of 3.2 ± 0.9 mm Hg, P = 0.01) and a subsequent significant drop in IOP to 12.4 ± 1.0 mm Hg after the injection of F4H5 (decrease of 5.3 ± 1.1 mm Hg, P < 0.01).
Emulsified silicone oil leads to blockage of the outflow pathways and an increased IOP in an in vitro human and porcine anterior segment perfusion model. With F4H5, a clinically relevant reversal of this increased outflow resistance was achieved and should be further evaluated.
本研究旨在利用人及猪眼前节的体外灌注模型，研究乳化硅油对小梁网流出阻力的影响。F4H5是一种已获批用于后节乳化硅油冲洗的药物，被评估为一种潜在的挽救治疗方法。
解剖17个猪眼前节和15个人眼前节，并夹入体外灌注模型。连续记录眼内压（IOP），并在注射乳化硅油前后进行评估，干预组随后用F4H5治疗。对照眼注射平衡盐溶液（BSS）。
在人眼中，注射乳化硅油使IOP从7.8±1.3毫米汞柱（mmHg）显著升高至15.8±3.5 mmHg（升高8.0±2.3 mmHg，P = 0.02，达到稳定较高压力的平均时间 = 8.6小时）。随后用F4H5治疗使IOP恢复至接近基线值（10.8±2.2 mmHg，与基线相比P = 0.06，达到稳定较低压力的平均时间 = 6.9小时）。猪眼呈现类似趋势，注射硅油后IOP从14.5±1.2 mmHg显著升高至17.7±1.6 mmHg（升高3.2±0.9 mmHg，P = 0.01），注射F4H5后IOP随后显著降至12.4±1.0 mmHg（降低5.3±1.1 mmHg，P < 0.01）。
在体外人及猪眼前节灌注模型中，乳化硅油导致流出途径阻塞和IOP升高。使用F4H5可实现这种增加的流出阻力的临床相关逆转，应进一步评估。
