使用体外灌注方法研究房水流出通道和眼压调节的共识性建议。
Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches.
作者信息
Acott Ted S, Fautsch Michael P, Mao Weiming, Ethier C Ross, Huang Alex S, Kelley Mary J, Aga Mini, Bhattacharya Sanjoy K, Borras Terete, Bovenkamp Diane, Chowdhury Uttio Roy, Clark Abbot F, Dibas Mohammed I, Du Yiqin, Elliott Michael H, Faralli Jennifer A, Gong Haiyan, Herberg Samuel, Johnstone Murray A, Kaufman Paul L, Keller Kate E, Kelly Ruth A, Krizaj David, Kuehn Markus H, Li Hoi Lam, Lieberman Raquel, Lin Shan C, Liu Yutao, McDonnell Fiona S, McDowell Colleen M, McLellan Gillian J, Mzyk Philip, Nair Kayarat Saidas, Overby Darryl R, Peters Donna M, Raghunathan VijayKrishna, Rao Ponugoti Vasantha, Roddy Gavin W, Sharif Najam A, Shim Myoung Sup, Sun Yang, Thomson Benjamin R, Toris Carol B, Willoughby Colin E, Zhang Hao F, Freddo Thomas F, Fuchshofer Rudolf, Hill Kamisha R, Karimi Alireza, Kizhatil Krishnakumar, Kopcyznski Casey C, Liton Paloma, Patel Gaurang, Peng Michael, Pattabiraman Padmanabhan P, Prasanna Ganesh, Reina-Torres Ester, Samples E Griffen, Samples John R, Steel Cynthia L, Strohmaier Clemens A, Subramanian Preeti, Sugali Chenna Kesavulu, van Batenburg-Sherwood Joseph, Wong Cydney, Youngblood Hannah, Zode Gulab S, White Elizabeth, Stamer W Daniel
机构信息
Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States.
Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States.
出版信息
Invest Ophthalmol Vis Sci. 2024 Dec 2;65(14):32. doi: 10.1167/iovs.65.14.32.
Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
眼压升高是青光眼性视神经病变进展的主要危险因素，也是目前主要的可治疗因素。除了直接的临床研究和活体动物体内研究外，眼前节和全眼球的离体灌注是研究传统房水流出功能的关键技术，因为它负责眼压调节。我们介绍了几种用于研究眼压调节的离体模型系统经过充分测试的实验细节、方案、注意事项、优点和局限性。这些包括：（1）灌注全眼球，（2）固定眼前节器官培养，（3）灌注人眼前节器官培养，（4）灌注动物眼前节器官培养，（5）灌注人角膜缘，以及（6）灌注人眼前节楔形组织。这些方法，在充分考虑其优势和局限性的情况下，构成了一套非常强大的工具，有助于扩展我们对眼压调节的理解。
Zotero 插件