de Sousa Bruna Rodrigues, de Melo Camylla Carvalho, Oliveira Manoel Marques Evangelista, de Lemos Hinrichsen Sylvia Maria, de Aguiar Cordeiro Rossana, de Lima-Neto Reginaldo Gonçalves
Tropical Medicine, Center for Medical Sciences, Federal University of Pernambuco (UFPE), Recife, Brazil.
Fungal Biology, Center of Biosciences, Federal University of Pernambuco (UFPE), Recife, Brazil.
Future Microbiol. 2025 Aug 8:1-7. doi: 10.1080/17460913.2025.2544485.
We evaluated the activity of isavuconazole against different clinical species from Brazil, with an emphasis on , using CLSI and EUCAST, and compared the results to expand the literature on this new triazole.
A total of 102 strains of spp. were isolated from critically ill patients admitted to tertiary hospitals in Recife, Pernambuco, Brazil. All isolates were identified using MALDI-TOF MS and tested using broth microdilutions.
The species identified were (62), (14), (9), (5), (3), , (2) each, , , , , and (1). A modal MIC of ≤0.008 µg/mL and a wild-type modal upper limit value of <0.03 µg/mL were found. Thus, 99.1% (CLSI) and 95.1% (EUCAST) of the strains were wild-type. The overall essential agreement rate between the methods was 95.1% (±2 log dilutions) and 89.2% (±1 log dilution).
Both methodologies were useful for evaluating the antifungal potential of isavuconazole and highlighted the low MICs of this triazole against the Brazilian collection of spp., especially the emerging yeast .
我们使用美国临床和实验室标准协会（CLSI）和欧洲抗菌药物敏感性试验委员会（EUCAST）的标准，评估了艾沙康唑对来自巴西的不同临床菌株的活性，重点是[具体菌株未明确写出]，并比较结果以扩充关于这种新型三唑类药物的文献。
从巴西伯南布哥州累西腓的三级医院收治的重症患者中分离出总共102株[具体菌株未明确写出]。所有分离株均使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行鉴定，并使用肉汤微量稀释法进行测试。
鉴定出的菌株包括[具体菌株未明确写出]（62株）、[具体菌株未明确写出]（14株）、[具体菌株未明确写出]（9株）、[具体菌株未明确写出]（5株）、[具体菌株未明确写出]（3株），[具体菌株未明确写出]、[具体菌株未明确写出]（各2株），[具体菌株未明确写出]、[具体菌株未明确写出]、[具体菌株未明确写出]、[具体菌株未明确写出]、[具体菌株未明确写出]和[具体菌株未明确写出]（各1株）。发现模态最低抑菌浓度（MIC）≤0.008μg/mL，野生型模态上限值<0.03μg/mL。因此，99.1%（CLSI）和95.1%（EUCAST）的菌株为野生型。两种方法之间的总体基本一致率为95.1%（±2倍对数稀释）和89.2%（±1倍对数稀释）。
两种方法都有助于评估艾沙康唑的抗真菌潜力，并突出了这种三唑类药物对巴西收集的[具体菌株未明确写出]菌株，特别是新兴酵母菌的低MIC值。
