Hatami Maryam, Masoudi Reza, Asadzadeh Nader, Sharafi Mohsen
Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Anim Reprod Sci. 2025 Sep;280:107967. doi: 10.1016/j.anireprosci.2025.107967. Epub 2025 Aug 6.
Sperm cryopreservation is a widely used procedure for storing donor semen prior to artificial insemination. However, spermatozoa experience biochemical and functional changes during cryopreservation. The current study aimed to compare the effects of mitochondrial-targeted (MitoQ and Mito-TEMPO) and untargeted (SOD and CoQ) antioxidants on the quality traits of ram sperm and reproductive performance. Semen samples were collected and diluted in extenders as follows: the first group served as the control, while the other groups were supplemented with 100 U/mL superoxide dismutase (SOD), 1 μM CoQ (Q10), 50 and 100 nM MitoQ (MQ50 and MQ100), 50 and 100 μM Mito-TEMPO (MT50 and MT100), and 50 nM MitoQ+ 50 μM Mito-TEMPO (MQ/MT). After the freezing, sperm quality characteristics and fertility potential were evaluated. According to the current results, the groups MQ50, MQ100, MT50, MT100, and MQ/MT exhibited higher (P ≤ 0.05) rates of total motility (TM), progressive motility (PM), average path velocity (VAP), membrane integrity (MI), mitochondrial activity potential (MAP), acrosome integrity (AI), viable cells, and fertility potential, as well as lower (P ≤ 0.05) late apoptotic-like changes, DNA fragmentation, HO concentration, and malondialdehyde (MDA) concentrations compared to the SOD and Q10 groups. Moreover, superoxide dismutase (SOD) concentration increased (P ≤ 0.05) in the groups of SOD, MT50, MT100, and MQ/MT than in other groups. Furthermore, the MQ/MT group exhibited the highest (P ≤ 0.05) rates of TM, PM, VAP, MI, MAP, AI, viable cells, and fertility potential, along with the lowest (P ≤ 0.05) late apoptotic-like changes, DNA fragmentation, HO concentration, and MDA concentrations relative to the other groups. In conclusion, incorporating MitoQ+Mito-TEMPO into the extender during the semen freezing could be a valuable strategy to enhance the success of ram frozen semen.
精子冷冻保存是人工授精前储存供体精液的一种广泛应用的方法。然而，精子在冷冻保存过程中会经历生化和功能变化。本研究旨在比较线粒体靶向抗氧化剂（MitoQ和Mito-TEMPO）和非靶向抗氧化剂（超氧化物歧化酶（SOD）和辅酶Q（CoQ））对公羊精子质量性状和繁殖性能的影响。收集精液样本并按以下方式在稀释液中稀释：第一组作为对照，其他组分别添加100 U/mL超氧化物歧化酶（SOD）、1 μM辅酶Q（Q10）、50和100 nM MitoQ（MQ50和MQ100）、50和100 μM Mito-TEMPO（MT50和MT100）以及50 nM MitoQ + 50 μM Mito-TEMPO（MQ/MT）。冷冻后，评估精子质量特征和生育潜力。根据当前结果，与SOD和Q10组相比，MQ50、MQ100、MT50、MT100和MQ/MT组的总活力（TM）、前向运动率（PM）、平均路径速度（VAP）、膜完整性（MI）、线粒体活性潜力（MAP）、顶体完整性（AI）、活细胞率和生育潜力更高（P≤0.05），而晚期凋亡样变化、DNA片段化、羟基自由基（HO）浓度和丙二醛（MDA）浓度更低（P≤0.05）。此外，SOD、MT50、MT100和MQ/MT组的超氧化物歧化酶（SOD）浓度比其他组增加（P≤0.05）。此外，相对于其他组，MQ/MT组的TM、PM、VAP、MI、MAP、AI、活细胞率和生育潜力最高（P≤0.05），而晚期凋亡样变化、DNA片段化、HO浓度和MDA浓度最低（P≤0.05）。总之，在精液冷冻过程中将MitoQ + Mito-TEMPO添加到稀释液中可能是提高公羊冷冻精液成功率的一种有价值的策略。
