Chen Haodong, Wang Junyi, Yang Songbai, Tang Jiawei, Tang Wen, Chen Shaoxin
National Key Laboratory of Lead Druggability Research, China State Institute of Pharmaceutical Industry, Shanghai Institute of Pharmaceutical Industry, Shanghai, 201203, China.
BMC Genom Data. 2025 Aug 8;26(1):55. doi: 10.1186/s12863-025-01348-9.
Plant endophytic fungusHormonema carpetanum is the only organism known to produce enfumafungin to date. Enfumafungin is a fernane-type triterpenoid glycoside compound with broad-spectrum antifungal activity, and this leading compound has been developed to antifungal agent ibrexafungerp approved by Food and Drug Administration (FDA). Here we report the first de novo assembled and annotated whole-genome sequence of H. carpetanum ATCC 74360, the original enfumafungin producing strain from the American Type Culture Collection (ATCC).
We sequenced the genome of H. carpetanum ATCC 74360 through a hybrid sequencing strategy combining Illumina NovaSeq X Plus short-read and PacBio Sequel IIe long-read technologies. Data from the two platforms were combined and the de novo assembly estimated a 32.93 Mbp genome divided into 16 contigs with a GC content of 49.85%. Genome annotation identified 10,433 genes, 253 tRNAs, and 62 rRNAs. antiSMASH predicted biosynthesis gene cluster (BGC) of secondary metabolite, including enfumafungin BGC. This genome and related data files presented here will provide a foundation for further gene editing of this important antibiotic-producing microorganism.
植物内生真菌地毯 Hormonema 是迄今为止已知唯一能产生恩夫马芬净的生物体。恩夫马芬净是一种具有广谱抗真菌活性的蕨烷型三萜糖苷化合物，这种先导化合物已被开发为美国食品药品监督管理局（FDA）批准的抗真菌药物依布列xafungerp。在此，我们报告了地毯 Hormonema ATCC 74360 的首个从头组装和注释的全基因组序列，该菌株是美国典型培养物保藏中心（ATCC）中最初产生恩夫马芬净的菌株。
我们通过结合 Illumina NovaSeq X Plus 短读长和 PacBio Sequel IIe 长读长技术的混合测序策略对地毯 Hormonema ATCC 74360 的基因组进行了测序。将来自两个平台的数据进行合并，从头组装估计该基因组大小为 32.93 Mbp，分为 16 个重叠群，GC 含量为 49.85%。基因组注释鉴定出 10433 个基因、253 个 tRNA 和 62 个 rRNA。antiSMASH 预测了次级代谢产物的生物合成基因簇（BGC），包括恩夫马芬净 BGC。这里呈现的这个基因组和相关数据文件将为对这种重要的抗生素生产微生物进行进一步的基因编辑提供基础。
