• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状RNA GCLC通过调控miR-516b-5p/GNAS轴促进骨髓间充质干细胞的成骨分化。

Circular RNA GCLC promotes osteogenic differentiation of bone marrow mesenchymal stem cells via modulating miR-516b-5p/GNAS axis.

作者信息

Li An, Zhang Sheng, Zhang Jing, Peng Zhihua, Feng Qinghui

机构信息

Deparment of Orthopedics and Traumatology, The Affiliated Traditional Chinese Medicine Hospital, Guangzhou Medical University, Guangzhou, 510000, China.

Medical Clinic, The Affiliated Traditional Chinese Medicine Hospital, Guangzhou Medical University, Guangzhou, 510000, China.

出版信息

J Orthop Surg Res. 2025 Aug 8;20(1):749. doi: 10.1186/s13018-025-06115-1.

DOI:10.1186/s13018-025-06115-1
PMID:40781640
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12333199/
Abstract

OBJECTIVE

Osteoporosis (OP) is a systemic bone disease without effective treatment at present. The aim of this study is to explore the role of the circular RNA GCLC (circGCLC) in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).

METHODS

The expression of circGCLC, miR-516b-5p, and the G protein alpha subunit (GNAS) was determined during osteogenic differentiation of BMSCs. Plasmid or oligonucleotide transfection was used to modify the expression levels of circGCLC, miR-516b-5p, and GNAS for assessing their effects on osteogenic differentiation ability, osteogenesis-related proteins, and MAPK signalling pathway-related proteins in BMSCs. The interactions among circGCLC, miR-516b-5p, and GNAS were also investigated.

RESULTS

During osteogenic differentiation of BMSCs, circGCLC and GNAS levels increased, whereas miR-516b-5p expression decreased. The knockdown of circGCLC inhibited osteogenic differentiation and MAPK signalling pathway activation in BMSCs. The increase in osteogenic differentiation that was induced by circGCLC upregulation was reversed by overexpressing miR-516b-5p. The inhibitory effects of circGCLC silencing on BMSC osteogenic differentiation were counteracted by GNAS upregulation. CircGCLC competitively bound to miR-516b-5p, mediating GNAS expression.

CONCLUSION

CircGCLC activates the MAPK signalling pathway by sponging miR-516b-5p, which mediates GNAS expression, thereby promoting the osteogenic differentiation of BMSCs.

摘要

目的

骨质疏松症（OP）是一种目前尚无有效治疗方法的全身性骨病。本研究旨在探讨环状RNA GCLC（circGCLC）在骨髓间充质干细胞（BMSC）成骨分化中的作用。

方法

在BMSC成骨分化过程中测定circGCLC、miR-516b-5p和G蛋白α亚基（GNAS）的表达。采用质粒或寡核苷酸转染来改变circGCLC、miR-516b-5p和GNAS的表达水平，以评估它们对BMSC成骨分化能力、成骨相关蛋白和丝裂原活化蛋白激酶（MAPK）信号通路相关蛋白的影响。还研究了circGCLC、miR-516b-5p和GNAS之间的相互作用。

结果

在BMSC成骨分化过程中，circGCLC和GNAS水平升高，而miR-516b-5p表达降低。敲低circGCLC可抑制BMSC的成骨分化和MAPK信号通路激活。过表达miR-516b-5p可逆转circGCLC上调诱导的成骨分化增加。上调GNAS可抵消circGCLC沉默对BMSC成骨分化的抑制作用。CircGCLC与miR-516b-5p竞争性结合，介导GNAS表达。

结论

CircGCLC通过吸附miR-516b-5p激活MAPK信号通路，miR-516b-5p介导GNAS表达，从而促进BMSC的成骨分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/0aa2f890e7c7/13018_2025_6115_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/5b0cea83015e/13018_2025_6115_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/4ea0c1f37d4f/13018_2025_6115_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/e0fea05b16ff/13018_2025_6115_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/a11cb8abb6c7/13018_2025_6115_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/d36ebd89e655/13018_2025_6115_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/0aa2f890e7c7/13018_2025_6115_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/5b0cea83015e/13018_2025_6115_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/4ea0c1f37d4f/13018_2025_6115_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/e0fea05b16ff/13018_2025_6115_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/a11cb8abb6c7/13018_2025_6115_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/d36ebd89e655/13018_2025_6115_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af75/12333199/0aa2f890e7c7/13018_2025_6115_Fig6_HTML.jpg

相似文献

1
Circular RNA GCLC promotes osteogenic differentiation of bone marrow mesenchymal stem cells via modulating miR-516b-5p/GNAS axis.环状RNA GCLC通过调控miR-516b-5p/GNAS轴促进骨髓间充质干细胞的成骨分化。
J Orthop Surg Res. 2025 Aug 8;20(1):749. doi: 10.1186/s13018-025-06115-1.
2
Circular RNA-FK501 binding protein 51 boosts bone marrow mesenchymal stem cell proliferation and osteogenic differentiation via modulating microRNA-205-5p/Runt-associated transcription factor 2 axis.环状 RNA-FK501 结合蛋白 51 通过调节 microRNA-205-5p/Runt 相关转录因子 2 轴促进骨髓间充质干细胞增殖和成骨分化。
J Orthop Surg Res. 2023 Oct 18;18(1):782. doi: 10.1186/s13018-023-04242-1.
3
Facilitative role of circPVT1 in osteogenic differentiation potentials of bone marrow mesenchymal stem cells from patients with osteoporosis through the miR-30d-5p/ITGB3 axis.环状 RNA（circRNA）PVT1 通过 miR-30d-5p/ITGB3 轴促进骨质疏松症患者骨髓间充质干细胞成骨分化潜能的作用。
Tissue Cell. 2022 Jun;76:101793. doi: 10.1016/j.tice.2022.101793. Epub 2022 Mar 31.
4
lncRNA IGF2-AS promotes the osteogenic differentiation of bone marrow mesenchymal stem cells by sponging miR-3,126-5p to upregulate KLK4.lncRNA IGF2-AS 通过海绵吸附 miR-3,126-5p 来上调 KLK4 促进骨髓间充质干细胞的成骨分化。
J Gene Med. 2021 Oct;23(10):e3372. doi: 10.1002/jgm.3372. Epub 2021 Jul 16.
5
Long noncoding RNA HCG18 inhibits the differentiation of human bone marrow-derived mesenchymal stem cells in osteoporosis by targeting miR-30a-5p/NOTCH1 axis.长链非编码 RNA HCG18 通过靶向 miR-30a-5p/NOTCH1 轴抑制骨质疏松症中人骨髓间充质干细胞的分化。
Mol Med. 2020 Nov 11;26(1):106. doi: 10.1186/s10020-020-00219-6.
6
The Inhibition of MicroRNA-139-5p Promoted Osteoporosis of Bone Marrow-Derived Mesenchymal Stem Cells by Targeting Wnt/Beta-Catenin Signaling Pathway by NOTCH1.NOTCH1 通过靶向 Wnt/β-联蛋白信号通路抑制微小 RNA-139-5p 促进骨髓间充质干细胞骨质疏松症。
J Microbiol Biotechnol. 2020 Mar 28;30(3):448-458. doi: 10.4014/jmb.1908.08036.
7
Circular RNA circ_0000020 promotes osteogenic differentiation to reduce osteoporosis via sponging microRNA miR-142-5p to up-regulate Bone Morphogenetic Protein BMP2.环状 RNA circ_0000020 通过海绵吸附 microRNA miR-142-5p 促进成骨分化，从而上调骨形态发生蛋白 BMP2 来减轻骨质疏松。
Bioengineered. 2021 Dec;12(1):3824-3836. doi: 10.1080/21655979.2021.1949514.
8
Circ-SLC8A1 regulates osteoporosis through blocking the inhibitory effect of miR-516b-5p on AKAP2 expression.环状 RNA-SLC8A1 通过阻断 miR-516b-5p 对 AKAP2 表达的抑制作用来调节骨质疏松症。
J Gene Med. 2020 Nov;22(11):e3263. doi: 10.1002/jgm.3263. Epub 2020 Sep 9.
9
mir-150-5p inhibits the osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting irisin to regulate the p38/MAPK signaling pathway.miR-150-5p 通过靶向鸢尾素调控 p38/MAPK 信号通路抑制骨髓间充质干细胞成骨分化。
J Orthop Surg Res. 2024 Mar 18;19(1):190. doi: 10.1186/s13018-024-04671-6.
10
Circular RNA circ-3626 promotes bone formation by modulating the miR-338-3p/Runx2 axis.环状 RNA circ-3626 通过调控 miR-338-3p/Runx2 轴促进成骨。
Joint Bone Spine. 2024 Mar;91(2):105669. doi: 10.1016/j.jbspin.2023.105669. Epub 2023 Nov 30.

本文引用的文献

1
Circular RNAs in the management of human osteoporosis.环状RNA在人类骨质疏松症管理中的作用
Br Med Bull. 2025 Jan 16;153(1). doi: 10.1093/bmb/ldae024.
2
Long non-coding and circular RNAs in osteoporosis: Translation to clinical practice.长非编码 RNA 和环状 RNA 在骨质疏松症中的作用：向临床实践的转化。
Adv Clin Chem. 2024;122:141-170. doi: 10.1016/bs.acc.2024.06.007. Epub 2024 Jun 25.
3
Epigenetic regulations of cellular senescence in osteoporosis.骨质疏松症中细胞衰老的表观遗传调控。
Ageing Res Rev. 2024 Aug;99:102235. doi: 10.1016/j.arr.2024.102235. Epub 2024 Feb 16.
4
Small interfering RNAs in the management of human osteoporosis.小干扰 RNA 在人类骨质疏松症治疗中的应用。
Br Med Bull. 2023 Dec 11;148(1):58-69. doi: 10.1093/bmb/ldad023.
5
Osteogenesis of bone marrow mesenchymal stem cell in hyperglycemia.高血糖症下骨髓间充质干细胞的成骨作用。
Front Endocrinol (Lausanne). 2023 Jun 21;14:1150068. doi: 10.3389/fendo.2023.1150068. eCollection 2023.
6
Circular RNAs: Promising Targets in Osteoporosis.环状 RNA：骨质疏松症的有前途靶点。
Curr Osteoporos Rep. 2023 Jun;21(3):289-302. doi: 10.1007/s11914-023-00786-7. Epub 2023 Apr 29.
7
Small interfering RNAs in the management of human rheumatoid arthritis.小干扰 RNA 在人类类风湿性关节炎治疗中的应用。
Br Med Bull. 2022 Jul 9;142(1):34-43. doi: 10.1093/bmb/ldac012.
8
Epidermal growth factor signalling pathway in endochondral ossification: an evidence-based narrative review.软骨内骨化过程中的表皮生长因子信号通路：基于证据的叙述性综述。
Ann Med. 2022 Dec;54(1):37-50. doi: 10.1080/07853890.2021.2015798.
9
High-throughput sequencing identified circular RNA circUBE2K mediating RhoA associated bladder cancer phenotype via regulation of miR-516b-5p/ARHGAP5 axis.高通量测序鉴定出环状 RNA circUBE2K 通过调节 miR-516b-5p/ARHGAP5 轴介导 RhoA 相关膀胱癌表型。
Cell Death Dis. 2021 Jul 20;12(8):719. doi: 10.1038/s41419-021-03977-1.
10
Identification and comparison of novel circular RNAs with associated co-expression and competing endogenous RNA networks in postmenopausal osteoporosis.绝经后骨质疏松症相关新型环状 RNA 的鉴定与比较及其与共表达和竞争内源性 RNA 网络的关系。
J Orthop Surg Res. 2021 Jul 16;16(1):459. doi: 10.1186/s13018-021-02604-1.