Inoue Dan, Oda Takashi, Suzuki Tadaki, Kataoka Michiyo, Iwama Sachiko, Dohi Yuko, Konno Osamu, Yamada Muneharu, Takeuchi Hironori, Iwamoto Hitoshi
Department of Nephrology and Blood Purification, Kidney Disease Center, Tokyo Medical University Hachioji Medical Center, Hachioji, Tokyo, Japan.
Department of Pathology, National Institute of Infectious Diseases, Shinjuku-Ku, Tokyo, Japan.
Virchows Arch. 2025 Aug 9. doi: 10.1007/s00428-025-04214-2.
Parvovirus B19 (PVB19) is usually not a subject of pre-transplant evaluation. Little is known regarding the localization of PVB19 in normal kidneys, particularly in relation to its cellular receptor, the P antigen. Tissue specimens obtained from 0-h renal allograft biopsies were investigated for the presence of PVB19 DNA by polymerase chain reaction (PCR) and the relative localization of P antigen and PVB19-related protein by double immunofluorescence staining. Double staining of in situ hybridization for PVB19 DNA and immunofluorescence staining for PVB19-related proteins was also performed. The presence of the virus was further evaluated by electron microscopy in selected biopsy specimens. PCR analysis detected PVB19 DNA in 39 out of 112 (35%) tissue samples. Immunohistochemical analysis of these 39 PVB19 DNA-positive samples revealed that 28 (72%) exhibited positive staining for PVB19-related proteins within some tubular epithelial cells. Electron microscopy demonstrated regular polygonal virus-like particles within tubular epithelial cells. Furthermore, most PVB19-positive tubular epithelial cells expressed the P antigen on their apical surfaces. PVB19 DNA and PVB19-related proteins co-localized in some tubular epithelial cells. Thus, approximately 1/3 of healthy adult kidneys contain PVB19, which is localized mainly in distal tubules expressing P antigens on their apical surface. The concurrent detection of PVB19 DNA and proteins, along with the visualization of virus-like particles by electron microscopy, suggests that PVB19 could live with protein-producing capacity. Given the relatively high frequency of detection, the possibility of PVB19 infection should be carefully considered in the context of kidney transplantation.
细小病毒B19(PVB19)通常不是移植前评估的对象。关于PVB19在正常肾脏中的定位,尤其是与其细胞受体P抗原的关系,人们了解甚少。通过聚合酶链反应(PCR)检测从0小时肾移植活检获得的组织标本中PVB19 DNA的存在,并通过双重免疫荧光染色检测P抗原和PVB19相关蛋白的相对定位。还进行了PVB19 DNA原位杂交和PVB19相关蛋白免疫荧光染色的双重染色。通过电子显微镜对选定的活检标本进一步评估病毒的存在。PCR分析在112个组织样本中的39个(35%)中检测到PVB19 DNA。对这39个PVB19 DNA阳性样本的免疫组织化学分析显示,28个(72%)在一些肾小管上皮细胞中表现出PVB19相关蛋白的阳性染色。电子显微镜显示肾小管上皮细胞内有规则的多边形病毒样颗粒。此外,大多数PVB19阳性肾小管上皮细胞在其顶端表面表达P抗原。PVB19 DNA和PVB19相关蛋白在一些肾小管上皮细胞中共定位。因此,大约1/3的健康成人肾脏含有PVB19,其主要定位于顶端表面表达P抗原的远端小管。PVB19 DNA和蛋白的同时检测,以及通过电子显微镜观察到病毒样颗粒,表明PVB19可能具有产生蛋白的生存能力。鉴于检测频率相对较高,在肾移植的背景下应仔细考虑PVB19感染的可能性。