甜樱桃中AKR基因家族的鉴定及其对不同非生物胁迫的响应。

Identification of the AKR gene family in sweet cherry and its response to different abiotic stresses.

作者信息

Guo Zhigang, An Xiaojuan, Zou Yali, Deng Fei

机构信息

Gansu Province Cherry Technology Innovation Center, Tianshui Normal University, Tianshui City, Gansu Province, China.

出版信息

Sci Rep. 2025 Aug 10;15(1):29251. doi: 10.1038/s41598-025-14284-6.

DOI:10.1038/s41598-025-14284-6

PMID:40784903

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12336331/

Abstract

To explore the role of aldehyde-keto reductase (AKR) gene family in sweet cherry (Prunus avium L.) responses to abiotic stresses, we identified 38 PaAKR genes via bioinformatics and analyzed their expression under PEG6000 (drought), NaCl (salinity), and ABA (hormone) treatments. Evolutionary analysis classified these genes into 5 subfamilies, with cis-acting elements indicating involvement in stress and hormone signaling. Real-time PCR showed that 8 genes (PaAKR3, PaAKR6, PaAKR10, PaAKR12, PaAKR17, PaAKR24, PaAKR28, PaAKR34) were strongly responsive to all three treatments. These findings highlight the potential of PaAKRs in mediating abiotic stress adaptation in sweet cherry and provide key candidate genes for enhancing stress resistance through functional studies.

摘要

为了探究醛酮还原酶（AKR）基因家族在甜樱桃（Prunus avium L.）对非生物胁迫响应中的作用，我们通过生物信息学方法鉴定了38个PaAKR基因，并分析了它们在聚乙二醇6000（干旱）、氯化钠（盐度）和脱落酸（激素）处理下的表达情况。进化分析将这些基因分为5个亚家族，顺式作用元件表明它们参与胁迫和激素信号传导。实时荧光定量PCR显示，8个基因（PaAKR3、PaAKR6、PaAKR10、PaAKR12、PaAKR17、PaAKR24、PaAKR28、PaAKR34）对所有三种处理均有强烈响应。这些发现突出了PaAKR在介导甜樱桃非生物胁迫适应性方面的潜力，并为通过功能研究增强抗逆性提供了关键候选基因。