Wei Lin, Ren Xinman, Qin Lumin, Zhang Rong, Cui Minghan, Xia Guangmin, Liu Shuwei
Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
J Integr Plant Biol. 2025 Jan;67(1):19-34. doi: 10.1111/jipb.13793. Epub 2024 Oct 22.
Saline-alkaline soils are a major environmental problem that limit plant growth and crop productivity. Plasma membrane H-ATPases and the salt overly sensitive (SOS) signaling pathway play important roles in plant responses to saline-alkali stress. However, little is known about the functional genes and mechanisms regulating the transcription of H-ATPases and SOS pathway genes under saline-alkali stress. In the present study, we identified that the plant AT-rich sequence and zinc-binding (TaPLATZ2) transcription factor are involved in wheat response to saline-alkali stress by directly suppressing the expression of TaHA2/TaSOS3. The knockdown of TaPLATZ2 enhances salt and alkali stress tolerance, while overexpression of TaPLATZ2 leads to salt and alkali stress sensitivity in wheat. In addition, TaWRKY55 directly upregulated the expression of TaPLATZ2 during saline-alkali stress. Through knockdown and overexpression of TaWRKY55 in wheat, TaWRKY55 was shown to negatively modulate salt and alkali stress tolerance. Genetic analyses confirmed that TaPLATZ2 functions downstream of TaWRKY55 in response to salt and alkaline stresses. These findings provide a TaWRKY55-TaPLATZ2-TaHA2/TaSOS3 regulatory module that regulates wheat responses to saline-alkali stress.
盐碱土是限制植物生长和作物生产力的主要环境问题。质膜H⁺-ATP酶和盐过度敏感（SOS）信号通路在植物对盐碱胁迫的响应中发挥重要作用。然而，关于盐碱胁迫下调控H⁺-ATP酶和SOS通路基因转录的功能基因和机制知之甚少。在本研究中，我们鉴定出植物富含AT序列和锌结合（TaPLATZ2）转录因子通过直接抑制TaHA2/TaSOS3的表达参与小麦对盐碱胁迫的响应。TaPLATZ2的敲低增强了盐和碱胁迫耐受性，而TaPLATZ2的过表达导致小麦对盐和碱胁迫敏感。此外，在盐碱胁迫期间，TaWRKY55直接上调TaPLATZ2的表达。通过在小麦中敲低和过表达TaWRKY55，结果表明TaWRKY55对盐和碱胁迫耐受性起负调控作用。遗传分析证实，在响应盐和碱胁迫时，TaPLATZ2在TaWRKY55的下游发挥作用。这些发现提供了一个调控小麦对盐碱胁迫响应的TaWRKY55-TaPLATZ2-TaHA2/TaSOS3调控模块。
