Wu Yuqi, Shan Xueling, Zhao Ziyu, Jiang Ding, Yan Guoping, Wang Wenchang, Shiigi Hiroshi, Hu Zhibang, Chen Zhidong
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, 213164, People's Republic of China.
Changzhou Institute of Inspection Testing Standardization and Certification, Changzhou, 213001, China.
Mikrochim Acta. 2025 Aug 11;192(9):575. doi: 10.1007/s00604-025-07446-w.
A dual-ligand strategy was employed to construct an electrochemiluminescence (ECL) sensing platform based on dual-ligand Tb-MOF and AgPO@TCPP for sensitive detection of chloramphenicol (CAP), demonstrating its potential applications in environmental monitoring and food safety. The dual-ligand Tb-MOF can be used as a co-reactant promoter, facilitating the rapid electroreduction of SO to generate more oxidative mediator SO, thereby enhancing the luminescence efficiency of the luminescent material AgPO@TCPP. Furthermore, the integration of AgPO@TCPP emitter could effectively overcome the aggregation-caused quenching (ACQ) effect of TCPP and then increase the luminescence intensity by seven-fold, which was attributed to the efficient electron transfer and energy transfer between AgPO and TCPP. Additionally, ECL spectra and electron spin resonance (ESR) spectra were utilized to investigate the possible ECL mechanism of the dual-ligand Tb-MOF/AgPO@TCPP system. As a proof of concept, we successfully established an ECL biosensor for detecting CAP, which displayed a wide linear range (10 ~ 10 M) and low detection limit (8.29 × 10 M), demonstrating practical feasibility. This study introduces a dual-ligand strategy to enhance ECL signals, expands the application scope of dual-ligand lanthanide MOFs, and provides new insights into designing high-performance sensing platforms.
采用双配体策略构建了基于双配体Tb-MOF和AgPO@TCPP的电化学发光（ECL）传感平台，用于灵敏检测氯霉素（CAP），展示了其在环境监测和食品安全中的潜在应用。双配体Tb-MOF可作为共反应促进剂，促进SO的快速电还原以生成更多的氧化介质SO，从而提高发光材料AgPO@TCPP的发光效率。此外，AgPO@TCPP发光体的整合可有效克服TCPP的聚集诱导猝灭（ACQ）效应，进而使发光强度提高7倍，这归因于AgPO与TCPP之间有效的电子转移和能量转移。此外，利用ECL光谱和电子自旋共振（ESR）光谱研究了双配体Tb-MOF/AgPO@TCPP体系可能的ECL机制。作为概念验证，我们成功建立了一种用于检测CAP的ECL生物传感器，其显示出宽线性范围（10 ~ 10 M）和低检测限（8.29 × 10 M），证明了实际可行性。本研究引入了一种增强ECL信号的双配体策略，扩展了双配体镧系金属有机框架的应用范围，并为设计高性能传感平台提供了新的见解。
