Zhang Huixin, Zhao Rui, Zhang Feifei, Xia Jianfei, Wang Zonghua
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, China; School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao 266071, China.
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, China.
Food Chem. 2024 Aug 1;448:139003. doi: 10.1016/j.foodchem.2024.139003. Epub 2024 Mar 23.
Chloramphenicol (CAP) is known to be harmful to the environment and food, posing a threat to human health. Developing an effective and convenient method for detecting CAP is crucial. An electrochemiluminescence (ECL) biosensor has been designed for sensitive detection of CAP. The improved ECL behavior was attributed to the synergistic effect of N and P co-doped TiC-Apt1 (N, P-TiC-Apt1) nanoprobes and high intensity focused ultrasound (HIFU) pretreatment. The doping of N and P could improve the electrochemical performance of TiC. HIFU pretreatment generated more reactive oxygen species (ROS) in the luminol-O system. N, P-TiC could aggregate and catalyze ROS, causing an increase in ECL intensity. Furthermore, N, P-TiC as a carrier loaded more aptamer, which could recognize CAP with high specificity. The detection limit was 0.01 ng/mL. This biosensor has been successfully applied in milk and environmental water samples, highlighting its potential in the field of food and environmental analysis.
氯霉素（CAP）已知对环境和食品有害，对人类健康构成威胁。开发一种有效且便捷的CAP检测方法至关重要。已设计出一种用于灵敏检测CAP的电化学发光（ECL）生物传感器。ECL行为的改善归因于氮和磷共掺杂的碳化钛-适配体1（N，P-TiC-Apt1）纳米探针与高强度聚焦超声（HIFU）预处理的协同效应。氮和磷的掺杂可改善碳化钛的电化学性能。HIFU预处理在鲁米诺-O体系中产生更多的活性氧（ROS）。N，P-TiC可聚集并催化ROS，导致ECL强度增加。此外，N，P-TiC作为载体负载了更多的适配体，其能够高度特异性地识别CAP。检测限为0.01 ng/mL。该生物传感器已成功应用于牛奶和环境水样中，凸显了其在食品和环境分析领域的潜力。
