Bempong M A, Montgomery C, Scully F E
J Environ Pathol Toxicol Oncol. 1985 Nov-Dec;6(2):241-51.
Chinese hamster ovary (CHO) cells were exposed to varying concentrations of N-chloropiperidine (NCP) for 3 or more hrs and harvested at different recovery periods for cytological analysis. Dose- and time-response curves for the cytostatic effects of NCP demonstrated a drastic reduction in percent cells with mitotic activity as NCP concentration increased. Analysis of control and treated cells for mitotic aberrations revealed that NCP-treated cells were characterized by abnormal nuclear division and distribution. The principal anomalies in nuclear division consisted of multipolar anaphase and ana-/telophase bridge-fragment configurations and laggards. Errors in nuclear distribution were mostly bi- and poly-macronucleation. Results of the cytogenetic studies showed that NCP-treated cells had a significant increase in chromosomal structural anomalies over those occurring in the control population. Exchange configurations, fragments and chromatid breaks were evident. CHO cell population exposed to NCP were also characterized by centromeric exaggeration.
将中国仓鼠卵巢（CHO）细胞暴露于不同浓度的N-氯代哌啶（NCP）中3小时或更长时间，并在不同的恢复期收获细胞用于细胞学分析。NCP细胞抑制作用的剂量-反应曲线和时间-反应曲线表明，随着NCP浓度的增加，有丝分裂活性细胞的百分比急剧下降。对对照细胞和处理细胞的有丝分裂畸变分析显示，经NCP处理的细胞的特征是核分裂和分布异常。核分裂中的主要异常包括多极后期、后期/末期桥-片段构型和落后染色体。核分布错误主要是双核和多核形成。细胞遗传学研究结果表明，与对照群体相比，经NCP处理的细胞的染色体结构异常显著增加。交换构型、片段和染色单体断裂很明显。暴露于NCP的CHO细胞群体的特征还包括着丝粒夸大。
