Bempong M A, Scully F E
J Environ Pathol Toxicol. 1980 Sep;4(2-3):345-54.
The toxicity and mutagenicity of N-chloropiperidine (NCP) and piperidine (PD) were tested in C57Bl/J6 mice and in Salmonella tester strains. Toxicity studies, based on single intraperitoneal administration of the test compounds, revealed that while the toxic effect of PD in aqueous solution decreased with time, the toxicity of aqueous solution of NCP increased on standing at room temperature for 24 or more hr. Direct incorporation assay of NCP and PD for mutagenic activity, using Salmonella tester strains as the test system, showed that the number of revertants induced by NCP was about 2.4 fold of that induced by PD. The results further indicated that TA100 and TA1535 were the most sensitive strains. A modified host-mediated assay, involving the analysis of urine, peritoneal fluid and faecal material from control and NCP-treated mice, indicated that peritoneal fluid from treated animals generated more revertants; moderate levels of revertants were produced by faecal material and urinary and urinary preparations produced the least number of revertants.
在C57Bl/J6小鼠和沙门氏菌测试菌株中对N-氯代哌啶（NCP）和哌啶（PD）的毒性和致突变性进行了测试。基于单次腹腔注射测试化合物的毒性研究表明，虽然PD水溶液的毒性随时间降低，但NCP水溶液在室温下放置24小时或更长时间后毒性增加。以沙门氏菌测试菌株为测试系统，对NCP和PD的诱变活性进行直接掺入试验，结果显示NCP诱导的回复突变体数量约为PD诱导数量的2.4倍。结果进一步表明，TA100和TA1535是最敏感的菌株。一种改良的宿主介导试验，涉及对对照小鼠和经NCP处理的小鼠的尿液、腹腔液和粪便物质进行分析，结果表明，处理动物的腹腔液产生的回复突变体更多；粪便物质产生中等水平的回复突变体，尿液及尿液制剂产生的回复突变体数量最少。
