A simple re-embedding method for the preparation of testicular cell cultures for light and electron microscopy.

A simple and reproducible method is described for the preparation of cell cultures for light and transmission electron microscopy. Mixed testicular cells are grown on collagen layered, PTFE-coated coverslips. Thin sheets of plastic-embedded cells are packed together and re-embedded permitting the examination of sections cut perpendicular to the plane of the culture and, thus, the intercellular relationships at different levels throughout the culture.