Li Zhifeng, Yang Yiwen, Tang Qiukai, Zeng Yanbo, Wang Hailong, Li Lei
Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China.
Clinical Laboratory, Zhejiang Sian International Hospital, Jiaxing, Zhejiang, 314031, China.
Mikrochim Acta. 2025 Aug 11;192(9):586. doi: 10.1007/s00604-025-07463-9.
A novel fluorescent organic compound, OBEI, exhibiting aggregation-induced emission enhancement (AIEE) characteristics was successfully synthesized. Leveraging its unique photophysical properties, a label-free fluorescent aptasensor was developed using OBEI as the fluorescent reporter and FeO@SiO magnetic beads as the adsorption substrate. The sensing mechanism relies on the formation of an INS-Apt/OBEI complex through electrostatic and hydrophobic interactions between OBEI and the insulin-specific aptamer (INS-Apt). This complex becomes immobilized on the magnetic bead surface via hydrogen bonding. Upon insulin introduction, the strong target-aptamer affinity triggers complex dissociation from the magnetic substrate, leaving the fluorescent INS-Apt/OBEI complex in solution after magnetic separation. The fluorescent intensity of the supernatant exhibited a linear response to insulin concentrations ranging from 0.033 pM to 10 nM, achieving a remarkable detection limit of 0.01 pM (S/N = 3). The biosensor demonstrates remarkable specificity for insulin against potential interferents and has been successfully implemented for insulin determination in human serum samples.
成功合成了一种具有聚集诱导发光增强（AIEE）特性的新型荧光有机化合物OBEI。利用其独特的光物理性质，以OBEI为荧光报告分子、FeO@SiO磁珠为吸附底物，开发了一种无标记荧光适配体传感器。传感机制依赖于OBEI与胰岛素特异性适配体（INS-Apt）之间通过静电和疏水相互作用形成INS-Apt/OBEI复合物。该复合物通过氢键固定在磁珠表面。引入胰岛素后，强大的靶标-适配体亲和力触发复合物从磁性底物上解离，磁分离后溶液中留下荧光INS-Apt/OBEI复合物。上清液的荧光强度对0.033 pM至10 nM的胰岛素浓度呈线性响应，检测限低至0.01 pM（S/N = 3）。该生物传感器对胰岛素具有显著的特异性，可有效抵抗潜在干扰物，已成功用于人血清样本中胰岛素的测定。
