Nandhakumar Ponnusamy, Sun Lei, Li Zhengxing, Cheung Christopher, Nguyen Ly, Ding Shichao, Gao Weiwei, Zhang Liangfang, Wang Joseph
Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California San Diego, La Jolla, California 92093, United States.
Anal Chem. 2024 Dec 10;96(49):19812-19821. doi: 10.1021/acs.analchem.4c05347. Epub 2024 Dec 2.
The growing need for reliable and rapid insulin testing to enhance glycemic management has spurred intensive exploration of new insulin-binding bioreceptors and innovative biosensing platforms for detecting this hormone, along with glucagon, in biological samples. Here, by leveraging the native protein receptors on the HepG2 cell membrane, we construct a simple and chemical-free biomimetic molecular recognition layer for the detection of insulin and glucagon. Unlike traditional affinity sensors, which require lengthy surface modifications on the electrochemical transducers and use of two different capture antibodies to recognize each analyte, this new biomimetic sensing strategy employs a simple drop-casting of a natural cell membrane recognition layer onto the electrochemical transducer. This approach allows for the concurrent capture and detection of both insulin and glucagon. We investigate the presence of insulin and glucagon receptors on the HepG2 cell membrane and demonstrate its multiplexing bioelectronic sensing capabilities through the binding of the captured insulin and glucagon to enzyme-tagged signaling antibodies. This new molecular recognition layer offers highly sensitive simultaneous detection of insulin and glucagon under decentralized conditions, holding considerable promise for the management of diabetes and the development of diverse biomimetic diagnostic platforms.
为加强血糖管理，对可靠且快速的胰岛素检测的需求日益增长，这促使人们深入探索用于在生物样品中检测这种激素以及胰高血糖素的新型胰岛素结合生物受体和创新生物传感平台。在此，通过利用HepG2细胞膜上的天然蛋白质受体，我们构建了一种用于检测胰岛素和胰高血糖素的简单且无化学物质的仿生分子识别层。与传统亲和传感器不同，传统亲和传感器需要在电化学换能器上进行冗长的表面修饰并使用两种不同的捕获抗体来识别每种分析物，这种新的仿生传感策略采用将天然细胞膜识别层简单滴铸到电化学换能器上的方法。这种方法能够同时捕获和检测胰岛素和胰高血糖素。我们研究了HepG2细胞膜上胰岛素和胰高血糖素受体的存在情况，并通过捕获的胰岛素和胰高血糖素与酶标记信号抗体的结合，展示了其多重生物电子传感能力。这种新的分子识别层在分散条件下能够高度灵敏地同时检测胰岛素和胰高血糖素，在糖尿病管理和各种仿生诊断平台的开发方面具有巨大潜力。
