Rezanejade Bardajee Ghasem, Rahimi Chahrogh Afsaneh, Monfared Aazam
Department of Polymer and Materials Chemistry, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, 19839-63113, Iran.
Department of Chemistry, Payame Noor University, Tehran, 19395-3697, Iran.
J Fluoresc. 2024 Aug 21. doi: 10.1007/s10895-024-03885-5.
Diabetes is a chronic metabolic disease characterized by high blood glucose (or blood sugar) levels, which harms the heart, blood vessels, eyes, kidneys, and nerves over time. So, it is crucial to regularly control glucose concentration in biological fluids to check its targets, reduce unpleasant symptoms of high and low blood sugar, and avoid long-term diabetes complications. This study developed a simple, rapid, sensitive, and cost-effective fluorescence system for glucose determination. The fluorescent Aptasensor was fabricated using cadmium telluride quantum dots (CdTe QDs) modified with thioglycolic acid and functionalized with thiol-glucose-aptamer through ligand exchange. The thiol-glucose-aptamer interacted directly with CdTe QDs, increasing fluorescence intensity. However, it decreased when the target molecules of glucose were introduced. The structural and morphological characteristics of the Aptasensor were confirmed by various analytical methods such as UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), energy dispersive x-ray spectroscopy (EDX), transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS). According to the typical Stern-Volmer equation, the relationship between fluorescent quenching and target concentration was linear with a detection limit (LOD) of 0.13 ± 1.95 × 10 mol L and a relative standard deviation (RSD) of 1.05%. The Aptasensor demonstrated high specificity towards the target and stability over 28 days. Furthermore, it detected glucose in human serum and urine with a recovery rate of up to 99.74%. The results indicate that the fluorescent Aptasensor could be valuable in developing robust sensing technology for low-concentrated analytes.
糖尿病是一种慢性代谢疾病，其特征是血糖（或血葡萄糖）水平升高，随着时间的推移会损害心脏、血管、眼睛、肾脏和神经。因此，定期控制生物体液中的葡萄糖浓度以检查其目标、减少高血糖和低血糖的不适症状并避免糖尿病长期并发症至关重要。本研究开发了一种用于葡萄糖测定的简单、快速、灵敏且经济高效的荧光系统。荧光适体传感器是使用巯基乙酸修饰的碲化镉量子点（CdTe QDs）并通过配体交换用硫醇-葡萄糖-适体功能化制备而成。硫醇-葡萄糖-适体直接与CdTe QDs相互作用，增加荧光强度。然而，当引入葡萄糖目标分子时荧光强度会降低。通过紫外可见光谱、傅里叶变换红外光谱（FT-IR）、场发射扫描电子显微镜（FESEM）、能量色散X射线光谱（EDX）、透射电子显微镜（TEM）、原子力显微镜（AFM）和动态光散射（DLS）等各种分析方法确认了适体传感器的结构和形态特征。根据典型的斯特恩-沃尔默方程，荧光猝灭与目标浓度之间的关系呈线性，检测限（LOD）为0.13±1.95×10 mol/L，相对标准偏差（RSD）为1.05%。该适体传感器对目标具有高特异性且在28天内具有稳定性。此外，它在人血清和尿液中检测葡萄糖的回收率高达99.74%。结果表明，荧光适体传感器在开发用于低浓度分析物的强大传感技术方面可能具有重要价值。
