Zheng Han, Xie Peng, Zheng Xiaoyu, Ma Xufa, Wei Kaijian, Han Rui
College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, College of Life Sciences, Hubei Normal University, Huangshi, 435002, China.
Sci Data. 2025 Aug 11;12(1):1400. doi: 10.1038/s41597-025-05779-y.
Phoxinus lagowskii is an economically important freshwater fish species native to China. In this study, a chromosome-level genome assembly of P. lagowskii was assembled using PacBio and Hi-C sequencing technologies. The total genome size was 1.22 Gb, with a contig N50 of 38.16 Mb. The final assembly comprised 25 chromosomes, with an anchor rate of 96.29%, resulting in a total anchored length of 1,173,227,270 bp. The genome completeness, as evaluated by the BUSCO assessment, was 94.2%. The genome contained 33,449 predicted protein-coding genes, and the BUSCO completeness for the gene set was 93.6%. A total of 76,416 proteins were predicted, of which 95.29% had functional annotations. Repetitive sequences in the genome totaled 626.50 Mb, representing 51.42% of the entire genome. This genome assembly of P. lagowskii provides significant insights into its biological traits, genetic diversity, and adaptive responses to environmental pressures. Moreover, it lays the groundwork for future genetic improvements in the aquaculture of this species.
拉氏鱥是一种原产于中国的具有重要经济价值的淡水鱼类。在本研究中，利用PacBio和Hi-C测序技术组装了拉氏鱥的染色体水平基因组。基因组总大小为1.22 Gb，重叠群N50为38.16 Mb。最终组装包含25条染色体，锚定率为96.29%，总锚定长度为1,173,227,270 bp。通过BUSCO评估，基因组完整性为94.2%。该基因组包含33,449个预测的蛋白质编码基因，基因集的BUSCO完整性为93.6%。总共预测了76,416个蛋白质，其中95.29%具有功能注释。基因组中的重复序列总计626.50 Mb，占整个基因组的51.42%。拉氏鱥的这种基因组组装为了解其生物学特性、遗传多样性以及对环境压力的适应性反应提供了重要见解。此外，它为该物种未来水产养殖中的遗传改良奠定了基础。
