Qu Shuo, Hu Shihao, Song Gengchen, Sun Haowen, Liu Fang, Zhang Miaoli, Zhan Yuhang, Li Yongguang, Teng Weili, Li Haiyan, Zhao Xue, Han Yingpeng
Key Laboratory of Soybean Biology in Chinese Ministry of Education (Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry), Northeast Agricultural University, Harbin, China.
Plant Genome. 2025 Sep;18(3):e70083. doi: 10.1002/tpg2.70083.
Soybean (Glycine max) is a major crop for grain and oil production worldwide. However, soybean cyst nematode (SCN) disease is a serious threat to soybean cultivation, causing major challenges for agriculture in China and globally. In this study, 306 soybean germplasms were evaluated for resistance to SCN HG type 0 using the female index (FI), a metric quantifying nematode reproduction on soybean roots. Furthermore, we performed a genome-wide association analysis study using 1,332,548 high-density single nucleotide polymorphism (SNP) markers. Through this analysis, the study identified quantitative trait nucleotides (QTN) and pinpointed candidate genes that are linked to resistance against SCN HG type 0. A total of 77 SNPs and 117 candidate genes associated with soybean resistance to SCN HG type 0 were identified. Subsequently, two kompetitive allele-specific PCR (KASP) markers, S19_rs8522772 and S19_rs8384176, along with four cleaved amplified polymorphic sequence (CAPS) markers (S19_rs838271, S19_rs8522589, S19_rs8466511 and S19_rs8481473) were developed. These markers were found to be closely linked to soybean resistance to SCN based on the results of beneficial allele analysis of candidate genes. These markers not only expanded the soybean germplasm resource, but also provided a solid foundation for molecular breeding of soybean varieties resistant to SCN.
大豆（Glycine max）是全球粮食和油料生产的主要作物。然而，大豆胞囊线虫（SCN）病对大豆种植构成严重威胁，给中国乃至全球农业带来重大挑战。在本研究中，利用雌性指数（FI）对306份大豆种质进行了抗SCN HG 0型评价，雌性指数是一种量化线虫在大豆根上繁殖情况的指标。此外，我们使用1332548个高密度单核苷酸多态性（SNP）标记进行了全基因组关联分析研究。通过该分析，本研究鉴定了数量性状核苷酸（QTN），并确定了与抗SCN HG 0型相关的候选基因。共鉴定出77个与大豆抗SCN HG 0型相关的SNP和117个候选基因。随后，开发了两个竞争性等位基因特异性PCR（KASP）标记，即S19_rs8522772和S19_rs8384176，以及四个酶切扩增多态性序列（CAPS）标记（S19_rs838271、S19_rs8522589、S19_rs8466511和S19_rs8481473）。基于候选基因的有利等位基因分析结果，发现这些标记与大豆对SCN的抗性密切相关。这些标记不仅扩大了大豆种质资源，也为抗SCN大豆品种的分子育种提供了坚实基础。
