Du Xijun, Hu Lin, Xu Weigang, Dong Haibin, Qi Xueli, Peng Chaojun, Zhang Yu, Li Zhengling
National Engineering Laboratory of Wheat, Key Laboratory for Wheat Germplasm Resources and Genetic Improvement, Key Laboratory of Wheat Biology and Genetic Breeding, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Henan Academy of Agricultural Sciences, Institute of Crops Molecular Breeding, Central Huanghuai Area, Zhengzhou, Henan Province, 450002, People's Republic of China.
The Shennong Laboratory, Zhengzhou, 450002, People's Republic of China.
BMC Plant Biol. 2025 Jul 2;25(1):808. doi: 10.1186/s12870-025-06918-8.
Historically, Henan Province has encountered multiple significant incidents of wheat cultivars resistance loss due to the emergence of new species of stripe rust fungus. To identify resistant germplasms exhibiting superior resistance to the emerging CRY34 strain, we employed a wheat 660 K array to genotype 256 Henan cultivars and conducted a genome-wide association study (GWAS) on adult plant resistance (APR) to wheat stripe rust in 2021-2022 and 2022-2023.
A total of 48 SNP markers were found to be significantly correlated with APR. Fifteen were consistently detected using multi-locus GWAS methods in both BLUP values and in at least one field season. Among these 15 stable quantitative trait nucleotides (QTNs), 12 were previously reported and three were novel. The major novel QTN QYr.hnk-7DL was located at chr07DL_AX-111,720,006. Twelve associated cultivars possessing this QTN demonstrated high APR to stripe rust, with a 4.69% favorable genotype frequency. The chr07DL_AX-111,720,006 SNP was developed into the KASP marker KASP-YrZM1354 for easier detection. The validity of QYr.hnk-7DL was confirmed using KASP-YrZM1354 in two natural populations and three F populations. Through gene expression analysis within the candidate interval region, TraesCS7D01G544100 (designated YrZM1354) was identified as the candidate gene for QYr.hnk-7DL.
we identified and verified the major QTN QYr.hnk-7DL associated with APR to stripe rust, elucidated the candidate gene YrZM1354 at this locus, and developed the high-precision marker KASP-YrZM1354. This marker has been successfully used in resistant germplasm screening and wheat breeding.
历史上，河南省曾因条锈菌新物种的出现而多次遭遇小麦品种抗性丧失的重大事件。为了鉴定对新出现的CRY34菌株表现出优异抗性的抗性种质，我们利用小麦660K芯片对256个河南品种进行基因分型，并在2021 - 2022年和2022 - 2023年对小麦条锈病成株抗性（APR）进行了全基因组关联研究（GWAS）。
共发现48个SNP标记与APR显著相关。使用多基因座GWAS方法在BLUP值和至少一个田间季节中一致检测到15个。在这15个稳定的数量性状核苷酸（QTN）中，12个先前已有报道，3个是新发现的。主要的新QTN QYr.hnk - 7DL位于chr07DL_AX - 111,720,006。拥有该QTN的12个相关品种对条锈病表现出高APR，有利基因型频率为4.69%。chr07DL_AX - 111,720,006 SNP被开发成KASP标记KASP - YrZM1354以便于检测。使用KASP - YrZM1354在两个自然群体和三个F群体中证实了QYr.hnk - 7DL的有效性。通过候选区间区域内的基因表达分析，TraesCS7D01G544100（命名为YrZM1354）被鉴定为QYr.hnk - 7DL的候选基因。
我们鉴定并验证了与条锈病APR相关的主要QTN QYr.hnk - 7DL，阐明了该位点的候选基因YrZM1354，并开发了高精度标记KASP - YrZM1354。该标记已成功用于抗性种质筛选和小麦育种。
