Ramatla Tsepo, Ncube Silence, Mokgokong Prudent, Nkhebenyane Jane, Molale-Tom Lesego, Ndou Rendani, Khasapane Ntelekwane, Bezuidenhout Carlos, Thekisoe Oriel, Lekota Kgaugelo
Centre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South Africa.
Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2531, South Africa.
One Health. 2025 Jul 30;21:101156. doi: 10.1016/j.onehlt.2025.101156. eCollection 2025 Dec.
The aim of this study was to investigate the prevalence and toxin type of isolated from broiler chicken faeces and determine its antibiotic resistance (AR) profile. A total of 480 broiler chicken faeces were collected from four different chicken abattoirs in North West Province, South Africa. Faecal samples were pooled (5 per pool from the same farm), resulting in 96 pooled samples. The disc diffusion method was used for documenting phenotypic AR, whilst PCR was used for the identification of and detection of toxin and antibiotic resistance genes. All 52 isolates identified as spp. using the gene PCR assay were also positive for the gene which is specific for . All 52 isolates harboured the gene responsible for encoding alpha toxin. Additionally, 7 (13.5 %) of these isolates were found to carry the gene. None of the isolates harboured , , and genes. All isolates in this study exhibited AR to ampicillin, followed by tetracycline, clindamycin, and chloramphenicol with resistance rates of 100 %, 71.15 %, 46.15 %, and 34.62 %, respectively. isolates contained tetracycline encoding genes, namely (A) and (W), chloramphenicol encoding genes which are: R, I, and II and beta-lactamase encoding genes including , , and . None of the isolates carried . This is the first study to characterize and determine its antimicrobial susceptibility phenotypically and genetically in food-producing chicken in South Africa, proving that animals may be sources of resistant strains of .
本研究的目的是调查从肉鸡粪便中分离出的[细菌名称未给出]的流行情况和毒素类型，并确定其抗生素耐药性（AR）谱。总共从南非西北省的四个不同鸡肉屠宰场收集了480份肉鸡粪便。粪便样本进行合并（每个样本池来自同一农场的5份样本），最终得到96个合并样本。采用纸片扩散法记录表型AR，同时使用PCR鉴定[细菌名称未给出]并检测毒素和抗生素耐药基因。使用[基因名称未给出]基因PCR检测法鉴定为[细菌名称未给出]属的所有52株分离株，对于特定于[细菌名称未给出]的[基因名称未给出]基因也呈阳性。所有52株分离株都携带负责编码α毒素的[基因名称未给出]基因。此外，这些分离株中有7株（13.5%）被发现携带[基因名称未给出]基因。没有分离株携带[基因名称未给出]、[基因名称未给出]和[基因名称未给出]基因。本研究中的所有分离株对氨苄青霉素均表现出耐药性，其次是四环素、克林霉素和氯霉素，耐药率分别为100%、71.15%、46.15%和34.62%。[细菌名称未给出]分离株含有四环素编码基因，即（A）和（W），氯霉素编码基因有：R、I和II，以及β-内酰胺酶编码基因，包括[基因名称未给出]、[基因名称未给出]、[基因名称未给出]和[基因名称未给出]。没有分离株携带[基因名称未给出]。这是第一项在南非对用于食品生产的鸡群中的[细菌名称未给出]进行表型和基因特征分析并确定其抗菌药敏性的研究，证明动物可能是[细菌名称未给出]耐药菌株的来源。
