Long-term survival of and in C3H/HeJ mice and their effect on Lyme arthritis and babesiosis manifestations.

is increasingly co-transmitted with Lyme disease-causing by ticks in the endemic regions of the United States. Infection in mice by this parasite mirrors human babesiosis, such as anemia, splenomegaly, alterations in splenic leukocyte balance, and diminished humoral immunity associated with enhanced Lyme disease manifestations at the acute phase. To evaluate the long-term survival of and in mice and their effects on pathogenesis, we conducted a 16-week infection experiment in C3H/HeJ mice. All mice infected with irrespective of the co-infection, displayed a low-level, albeit microscopically detectable, parasitemia in both male and female mice even after 16 weeks post-infection. Splenomegaly was detected at this stage in both male and female mice and was significantly higher in females infected solely with compared to co-infected mice, likely due to a greater peak parasitemia at the acute phase of infection and persistent splenic manifestations in these mice. Interestingly, disrupted the blood-brain barrier (BBB) in mice, as reported during cerebral malaria caused by . Furthermore, colonization could be detected until 16 weeks of infection, while more pronounced Lyme inflammatory arthritis was observed at 4 weeks post-infection. This study underscores the complex interactions between and to affect each disease, highlighting the potential implications for vaccine development against Lyme spirochetes and therapeutic management of co-infected individuals.IMPORTANCETick-borne co-infections are becoming increasingly prevalent worldwide due to simultaneous or sequential acquisition and transmission by species ticks during their blood meal. We reported that and co-infection reciprocally affects these pathogens during the acute phase of infection; however, the effect of co-infections on microbial long-term persistence in the murine model was not previously investigated. In this study, we have filled a critical lacuna in understanding the interactions between these two pathogens at different stages of infection and their effects on the host and disease manifestations in mice. Our investigation provides insights into their pathogenicity to allow the development of effective vaccines and successful antimicrobials against these tick-borne co-infections.