Girardin E, Paunier L
Magnesium. 1985;4(4):188-92.
Mg, K and Na were measured in erythrocytes, lymphocytes and serum obtained from 20 normal subjects. The cells of 20 ml of blood were separated in Ficoll gradient, washed with a choline buffer and digested with H2SO4. The cations were measured by atomic absorption photometry, protein was determined by the Lowry method and the results were expressed as nmol/mg of protein. Intracellular lymphocyte concentrations (mean +/- SD, n = 20) were: Mg: 57.6 +/- 5.1, Na: 30.0 +/- 7.9, K: 614.3 +/- 69. In the red cells, ion concentrations were: Mg: 7.97 +/- 0.8, Na: 20.9 +/- 4.8, K: 343.0 +/- 42.0. There was a highly significant correlation between Mg and K concentration in erythrocytes (p less than 0.001) and lymphocytes (p less than 0.001). A negative correlation (p less than 0.05) was also found between lymphocyte and erythrocyte K concentration and K serum concentration.
对20名正常受试者的红细胞、淋巴细胞和血清中的镁、钾和钠进行了测量。将20毫升血液的细胞在Ficoll梯度中分离，用胆碱缓冲液洗涤并用硫酸消化。通过原子吸收光度法测量阳离子，用Lowry法测定蛋白质，结果以每毫克蛋白质的纳摩尔数表示。细胞内淋巴细胞浓度（平均值±标准差，n = 20）为：镁：57.6±5.1，钠：30.0±7.9，钾：614.3±69。在红细胞中，离子浓度为：镁：7.97±0.8，钠：20.9±4.8，钾：343.0±42.0。红细胞（p<0.001）和淋巴细胞（p<0.001）中镁和钾浓度之间存在高度显著的相关性。淋巴细胞与红细胞钾浓度和血清钾浓度之间也存在负相关（p<0.05）。
