同时测定淋巴细胞、红细胞和血小板中的镁和钾。

Simultaneous determination of magnesium and potassium in lymphocytes, erythrocytes and thrombocytes.

作者信息

Schwinger R, Antoni D H, Guder W G

机构信息

Institut für Klinische Chemie, Krankenhaus München-Bogenhausen, Fed. Rep. of Germany.

出版信息

J Trace Elem Electrolytes Health Dis. 1987 Dec;1(2):89-98.

PMID:2856575

Abstract

A standardized method for the determination of magnesium and potassium in the lymphocytes, erythrocytes, and thrombocytes of normal subjects is described. The procedure consists of four steps: 1) the removal of platelets from the remaining cells and heparin plasma, 2) the isolation of platelet-poor lymphocytes by means of density-gradient centrifugation of the buffy-coat, 3) the purification of blood cells by repeated washings, and 4) the determination in cell lysates of magnesium by atomic absorption spectrometry and of potassium by flame photometry. In order to get reproducible lymphocyte magnesium values, it was necessary to obtain a mean lymphocyte recovery of 60% with a purity of 97% (nucleated cells), this yield containing less than 5 thrombocytes per lymphocyte and less than 4 erythrocytes per 100 lymphocytes. Under these conditions, the normal content of magnesium in lymphocytes was 3.5-5.7 fmoll/cell (n = 18), that of potassium was 38.8-63.5 fmol/cell. The precision (CV) of the whole procedure measured with 10 identical samples from each of 5 persons was 5.7% for lymphocyte magnesium and 7.8% for potassium, respectively. The concentration of magnesium in erythrocytes was 1.99-2.43 mmol/l and that of potassium was 85.8-102.5 mmol/l. The precision was 3.6% for magnesium and 2.2% for potassium. The content of magnesium in thrombocytes was found to be 0.07-0.12 fmol/cell.

摘要

本文描述了一种测定正常受试者淋巴细胞、红细胞和血小板中镁和钾的标准化方法。该方法包括四个步骤：1）从剩余细胞和肝素血浆中去除血小板；2）通过血沉棕黄层的密度梯度离心分离血小板缺乏的淋巴细胞；3）通过反复洗涤纯化血细胞；4）通过原子吸收光谱法测定细胞裂解液中的镁，通过火焰光度法测定钾。为了获得可重复的淋巴细胞镁值，必须使淋巴细胞平均回收率达到60%，纯度达到97%（有核细胞），该产量中每个淋巴细胞含血小板少于5个，每100个淋巴细胞含红细胞少于4个。在这些条件下，淋巴细胞中镁的正常含量为3.5 - 5.7 fmol/细胞（n = 18），钾的正常含量为38.8 - 63.5 fmol/细胞。用来自5个人的10个相同样本测量整个过程的精密度（CV），淋巴细胞镁为5.7%，钾为7.8%。红细胞中镁的浓度为1.99 - 2.43 mmol/L，钾的浓度为85.8 - 102.5 mmol/L。镁的精密度为3.6%；钾的精密度为2.2%。血小板中镁的含量为0.07 - 0.12 fmol/细胞。