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Measurement of ligand binding to proteins by fluorescence spectroscopy.

作者信息

Ward L D

出版信息

Methods Enzymol. 1985;117:400-14. doi: 10.1016/s0076-6879(85)17024-2.

DOI:10.1016/s0076-6879(85)17024-2
PMID:4079811
Abstract
摘要

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Measurement of ligand binding to proteins by fluorescence spectroscopy.通过荧光光谱法测量配体与蛋白质的结合
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Identification of the six ligands to manganese(II) in transition-state-analogue complexes of creatine kinase: oxygen-17 superhyperfine coupling from selectively labeled ligands.肌酸激酶过渡态类似物复合物中锰(II)的六种配体的鉴定:来自选择性标记配体的氧-17超精细偶合
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