人龈上牙石提取物中的一种硫酸化糖肽。

A sulphated glycopeptide in human supragingival calculus extracts.

作者信息

Embery G

出版信息

Calcif Tissue Res. 1977 May 31;23(1):13-7. doi: 10.1007/BF02012761.

DOI:10.1007/BF02012761

Abstract

A method is described for the isolation and purification of a sulphated glycopeptide from human supragingival calculus. The compound was isolated after using EDTA treatment, 2 M CaCl2 extraction, proteolytic digestion, ethanol precipitation, and finally purified by DEAE cellulose chromatography. It migrated as a single component of cellulose acetate electrophoresis, and chemical and infrared spectral analysis showed the presence of covalently attached sulphate groups. The sulphated glycopeptide was distinguished from being a sulphated glycosaminoglycan.

摘要

描述了一种从人龈上牙石中分离和纯化硫酸化糖肽的方法。该化合物经EDTA处理、2M氯化钙提取、蛋白水解消化、乙醇沉淀后分离，最后通过DEAE纤维素色谱法纯化。它在醋酸纤维素电泳中作为单一成分迁移，化学和红外光谱分析表明存在共价连接的硫酸基团。该硫酸化糖肽与硫酸化糖胺聚糖不同。