一种从组织中微量分离硫酸化糖肽的方法。

A method for micro-scale isolation of sulfated glycopeptide from tissue.

作者信息

Endo M, Yosizawa Z

出版信息

Tohoku J Exp Med. 1979 Nov;129(3):225-31. doi: 10.1620/tjem.129.225.

DOI:10.1620/tjem.129.225

Abstract

Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of pronase digestion (Step 1), cetylpyridinium chloride-fractionation (Step 2), crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment (Step 3) and preparative cellulose acetate membrane-electrophoresis (Step 4). By this method, sulfated glycopeptide was obtained in a high yield from estrogen-treated rabbit uterus.

摘要

通过依次进行链霉蛋白酶消化（步骤1）、十六烷基氯化吡啶分级分离（步骤2）、粗制肝素酶消化或软骨素酶ABC消化加亚硝酸处理（步骤3）以及制备性醋酸纤维素膜电泳（步骤4），实现了从组织中微量分离硫酸化糖肽。通过这种方法，从雌激素处理的兔子宫中以高产率获得了硫酸化糖肽。