Lesser M, Chang J C, Orlowski M
Mol Cell Biochem. 1985 Nov;69(1):67-73. doi: 10.1007/BF00225928.
Highly sensitive and specific synthetic substrates were used to quantitate cathepsin B and D activity in peritoneal macrophages in response to stimulation in vivo with mineral oil and thioglycollate. After intraperitoneal instillation of mineral oil the activity of cathepsin B increased significantly (to 15 300 units/mg protein versus 7 340 in saline controls), reaching values approaching those found in alveolar macrophages (18 400 units/mg protein). Significantly greater stimulation of enzyme activity was obtained after intraperitoneal instillation of thioglycollate (23 600 units/mg protein). Cathepsin D activity also increased significantly after both mineral oil and thioglycollate. However, the increase was moderate (from 806 to about 1 200 units/mg protein), remaining still more than six times lower than in alveolar macrophages. The data are the first to demonstrate that cathepsin B activity can be stimulated in vivo in peritoneal macrophages by instillation of agents that induce acute inflammation. They also point to a differential control of expression of cathepsin B and D activity in both peritoneal and alveolar macrophages in spite of the common lysosomal origin of the two enzymes.
使用高灵敏度和特异性的合成底物来定量腹腔巨噬细胞中组织蛋白酶B和D的活性，这些巨噬细胞是在体内经矿物油和巯基乙酸盐刺激后的反应。腹腔内注入矿物油后，组织蛋白酶B的活性显著增加（达到15300单位/毫克蛋白质，而生理盐水对照组为7340单位/毫克蛋白质），达到接近肺泡巨噬细胞中发现的值（18400单位/毫克蛋白质）。腹腔内注入巯基乙酸盐后，酶活性获得了显著更大的刺激（23600单位/毫克蛋白质）。在注入矿物油和巯基乙酸盐后，组织蛋白酶D的活性也显著增加。然而，增加幅度适中（从806单位/毫克蛋白质增加到约1200单位/毫克蛋白质），仍比肺泡巨噬细胞中的活性低六倍多。这些数据首次证明，通过注入诱导急性炎症的试剂，可在体内刺激腹腔巨噬细胞中的组织蛋白酶B活性。它们还指出，尽管这两种酶具有共同的溶酶体起源，但在腹腔和肺泡巨噬细胞中，组织蛋白酶B和D活性的表达存在差异调控。
