Wang Rongsheng, Lv Tan, Li Jingpeng, Ma Juntao, Wang Yongli, Deng Lingwei, Li Wan, Zhang Jun, Li Kun, Zhang Wei, Mu Fengchen, Zhang Guomin
Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
Heilongjiang Laboratory of Crop and Livestock Molecular Breeding, Harbin, Heilongjiang, China.
Front Genet. 2025 Jul 29;16:1617034. doi: 10.3389/fgene.2025.1617034. eCollection 2025.
China is the world's third largest saline-alkali land country, and the breeding of salt-tolerant rice varieties has always been a key focus of rice breeders. Screening and identifying salt-tolerant varieties and exploring related genes are essential for breeding.
In this study, 450 high-latitude resource populations were planted on natural saline-alkali soil for 2 years under 2 treatments. The comprehensive agronomic traits of the populations were evaluated. The principal component and cluster analyses were used to preliminarily group the phenotypes, and individual phenotypes were comprehensively scored and ranked to identify the top 40 saline-alkali tolerant varieties each year.
Notably, S321 and S19 were the most saline-alkali tolerant varieties each year. Genome-wide association studies identified one saline-alkali-related position near 6,636,119 bp on chromosome 8 and another near 23,311,931 bp on chromosome 11. Os08g0214233 and Os11g0604900 were the nearest genes from the identified positions, respectively. Gene annotation was used to further screen the polymorphic sites in the associated regions, identifying 17 and 48 genes with 593 variants, including 56 polymorphic sites located in exons.
This study provided candidate gene loci for the fine mapping of saline-alkali tolerance genes and offered excellent resistant rice resources for the molecular improvement of varieties.
中国是世界第三大盐碱地国家，耐盐碱水稻品种的培育一直是水稻育种者的重点关注对象。筛选和鉴定耐盐碱品种并探索相关基因对于育种至关重要。
在本研究中，450个高纬度资源群体在两种处理下于天然盐碱土上种植了两年。对群体的综合农艺性状进行了评估。采用主成分分析和聚类分析对表型进行初步分组，并对个体表型进行综合评分和排名，以每年鉴定出前40个耐盐碱品种。
值得注意的是，S321和S19每年都是耐盐碱能力最强的品种。全基因组关联研究在8号染色体上6,636,119 bp附近和11号染色体上23,311,931 bp附近分别鉴定出一个与盐碱相关的位点。Os08g0214233和Os11g0604900分别是距鉴定出的位点最近的基因。利用基因注释进一步筛选相关区域的多态性位点，鉴定出17个和48个基因，共593个变异，其中包括56个位于外显子的多态性位点。
本研究为耐盐碱基因的精细定位提供了候选基因位点，并为品种的分子改良提供了优良的抗性水稻资源。
