Kochetkov S N, Abduragimov A R, Gabibov A G, Severin E S
Mol Biol (Mosk). 1985 Nov-Dec;19(6):1562-8.
The catalytic subunit of cAMP-dependent protein kinase from pig brain was shown to catalyse an isotope exchange reaction ATP in equilibrium with ADP. The kinetic parameters of the exchange were determined. The enzyme can also use GTP as the donor substrate; phosphotransferase and "GTPase" reactions were investigated. Based on the kinetic data obtained in this and in the previous paper the free energy profiles of protein kinase catalysed reactions are discussed.
已证明猪脑中环磷酸腺苷（cAMP）依赖性蛋白激酶的催化亚基可催化ATP与ADP处于平衡状态下的同位素交换反应。测定了该交换反应的动力学参数。该酶还可以使用GTP作为供体底物；对磷酸转移酶和“GTP酶”反应进行了研究。基于本文及前一篇论文中获得的动力学数据，讨论了蛋白激酶催化反应的自由能概况。
