Hagen Simen, Jacques Corentin, Ranta Radu, Koessler Laurent, Maillard Louis, Colnat-Coulbois Sophie, Rossion Bruno, Jonas Jacques
Université de Lorraine, CNRS, IMoPA, Nancy, France.
Université de Lorraine, CNRS, CRAN, Nancy, France.
Imaging Neurosci (Camb). 2025 Feb 24;3. doi: 10.1162/imag_a_00479. eCollection 2025.
Intracranial EEG (iEEG) is increasingly used in many fields of human cognitive neuroscience since it offers a unique opportunity to directly record brain activity from awake humans at a high spatial and temporal resolution. However, little is known about the influence of the reference montage on the spatial and temporal characteristics of iEEG activity. Here, we compare the spatial and temporal profiles of neural activity for five reference montages (scalp reference, common average, zero reference, local Bipolar, and Laplacian) applied to a large dataset of depth electrodes (StereoElectroEncephaloGraphy, SEEG) recordings across the human ventral occipito-temporal cortex (VOTC, N individual brains = 77). Frequency-tagging is used for objective identification and quantification of both low- (<30 Hz) and high-frequency (40-160 Hz) face-selective neural activity. For low-frequency responses, similar spatial distributions and time-courses of significant face-selective contacts and of face-selective amplitudes are found across the five reference montages, although the latter two local reference montages enhance face selectivity along the fusiform gyrus until the anterior temporal lobe. However, they also reduce the right hemisphere dominance, a hallmark of face-selective neural activity, and increase the number of significant contacts in the white matter. For high-frequency responses, similar spatial distributions and time-courses of significant face-selective contacts and of face-selective amplitudes are found for all references, except for the scalp reference (SCA), which enhances face selectivity in lateral and medial regions of the anterior VOTC. However, SCA also increases the number of significant contacts in the white matter. Thus, specificities of each electrode montage should be considered before choosing an iEEG reference, according to the research question, the anatomical region, the type of analyses, and the responses frequency range.
颅内脑电图（iEEG）在人类认知神经科学的许多领域中得到越来越广泛的应用，因为它提供了一个独特的机会，可以在高空间和时间分辨率下直接记录清醒人类的大脑活动。然而，关于参考导联对iEEG活动的空间和时间特征的影响，我们知之甚少。在这里，我们比较了应用于人类腹侧枕颞叶皮层（VOTC，N个个体大脑=77）深度电极（立体脑电图，SEEG）记录的大型数据集的五种参考导联（头皮参考、公共平均、零参考、局部双极和拉普拉斯）的神经活动的空间和时间分布。频率标记用于客观识别和量化低频（<30Hz）和高频（40-160Hz）面部选择性神经活动。对于低频反应，在五种参考导联中发现了显著的面部选择性触点和面部选择性振幅的相似空间分布和时间进程，尽管后两种局部参考导联增强了沿梭状回直至颞叶前部的面部选择性。然而，它们也降低了右半球优势，这是面部选择性神经活动的一个标志，并增加了白质中显著触点的数量。对于高频反应，除头皮参考（SCA）外，所有参考导联的显著面部选择性触点和面部选择性振幅的空间分布和时间进程相似，头皮参考增强了前VOTC外侧和内侧区域的面部选择性。然而，SCA也增加了白质中显著触点的数量。因此，在根据研究问题、解剖区域、分析类型和反应频率范围选择iEEG参考之前，应考虑每个电极导联的特异性。
