影响黑腹果蝇核糖体基因染色体整合的染色体重排。
Chromosomal rearrangements which affect the chromosomal integration of the ribosomal genes in Drosophila melanogaster.
作者信息
Zuchowski C I, Harford A G
出版信息
Cell. 1977 Jun;11(2):383-8. doi: 10.1016/0092-8674(77)90055-1.
Sucrose gradient analysis of DNA from detergent-pronase lysates of whole adult flies has been used to examine a variety of genotypes for the presence of ribosomal genes not integrated into the DNA of the chromosome. Such genes were found in females in which one X chromosome carries an inversion, having one of its breakpoints between the nucleolus organizer and the centromere. These inversions move the nucleolus organizer to the distal end of the X chromosome. Other inversions which do not move the nucleolus organizer, as well as a series of bobbed deficiencies, did not induce unintegrated genes. The same inversions which induce unintegrated genes in adults also produce them in the diploid brain and imaginal discs of larvae. On the other hand, in the polytene salivary glands, unintegrated genes were found in every genotype examined.
利用成年果蝇全虫去污剂-链霉蛋白酶裂解物中的DNA进行蔗糖梯度分析，以检测各种基因型中是否存在未整合到染色体DNA中的核糖体基因。在雌性果蝇中发现了这类基因，其中一条X染色体发生了倒位，其一个断点位于核仁组织区和着丝粒之间。这些倒位将核仁组织区移到了X染色体的远端。其他未移动核仁组织区的倒位以及一系列截毛缺失，均未诱导出未整合基因。在成体中诱导出未整合基因的相同倒位，在幼虫的二倍体脑和成虫盘也会产生这类基因。另一方面，在多线唾液腺中，在所检测的每种基因型中都发现了未整合基因。
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