黑腹果蝇多线染色体中核糖体基因复制的独立控制。
Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster.
作者信息
Spear B B, Gall J G
出版信息
Proc Natl Acad Sci U S A. 1973 May;70(5):1359-63. doi: 10.1073/pnas.70.5.1359.
Abstract
The saturation values for ribosomal RNA hybridization have been determined for DNA from diploid and polytene tissues of Drosophila melanogaster. These values have been measured in XO and XX larvae which have, respectively, one and two nucleolus organizers in the diploid chromosome set. The results show that (1) in diploid cells the ribosomal (r)DNA is present in amounts proportional to the number of nucleolus organizers, (2) in polytene cells the rDNA is under-replicated with respect to the euchromatic DNA, and (3) in polytene cells the amount of rDNA is independent of the diploid number of nucleolus organizers. These observations suggest that somatic variations in rDNA content may involve independent polytenization of the nucleolus organizer without change in the number of ribosomal cistrons per organizer. The independent polytenization of rDNA is proposed as the chromosomal explanation for the relative increase in rDNA in flies of the XO constitution.
摘要
已测定了黑腹果蝇二倍体和多线组织DNA的核糖体RNA杂交饱和值。这些值是在XO和XX幼虫中测量的，它们在二倍体染色体组中分别有一个和两个核仁组织区。结果表明：（1）在二倍体细胞中，核糖体（r）DNA的含量与核仁组织区的数量成正比；（2）在多线细胞中，rDNA相对于常染色质DNA复制不足；（3）在多线细胞中，rDNA的量与核仁组织区的二倍体数量无关。这些观察结果表明，rDNA含量的体细胞变异可能涉及核仁组织区的独立多线化，而每个组织区的核糖体顺反子数量不变。rDNA的独立多线化被认为是XO型果蝇中rDNA相对增加的染色体解释。
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