嗜铬粒蛋白基质的糖蛋白：利用凝集素印迹法区分几个不同类别。

Glycoproteins of the chromaffin-granule matrix: use of lectin blotting to distinguish several separate classes.

作者信息

Apps D K, Phillips J H, Purves F C

出版信息

Neuroscience. 1985 Oct;16(2):477-87. doi: 10.1016/0306-4522(85)90019-3.

Abstract

The soluble proteins released by hypotonic lysis of highly purified bovine adrenal chromaffin granules were analysed by one- and two-dimensional electrophoresis, followed by transfer to nitrocellulose and decoration with lectins or specific antibodies. The effects of neuraminidase treatment, and of chemical deglycosylation by trifluoromethanesulphonic acid, were investigated. It was shown that lectins could be used to distinguish the two major series of chromogranins from each other, from dopamine beta-hydroxylase and from several minor, unidentified glycoprotein components of the lysate. Antibody decoration revealed a complex series of peptides containing enkephalin sequences, some of which changed their electrophoretic mobility on treatment with trifluoromethanesulphonic acid.

摘要

通过一维和二维电泳分析高纯度牛肾上腺嗜铬颗粒经低渗裂解后释放的可溶性蛋白质，随后转移至硝酸纤维素膜并用凝集素或特异性抗体进行标记。研究了神经氨酸酶处理以及三氟甲磺酸化学去糖基化的效果。结果表明，凝集素可用于区分两种主要的嗜铬粒蛋白系列，以及将它们与多巴胺β-羟化酶和裂解物中几种未鉴定的次要糖蛋白成分区分开来。抗体标记揭示了一系列含有脑啡肽序列的复杂肽段，其中一些在经三氟甲磺酸处理后其电泳迁移率发生了变化。