Xun Pei, Zong Jiabao, Li Shenglan, Li Wenbin
Department of Neuro-Oncology , Cancer Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Department of Oncology, Beijing Daxing District People's Hospital, Beijing, China.
Future Sci OA. 2025 Dec;11(1):2546232. doi: 10.1080/20565623.2025.2546232. Epub 2025 Aug 13.
To investigate whether liposomal honokiol enhances the anti-tumor effect of bevacizumab (BEV) in glioblastoma (GBM) and explore its underlying mechanism.
MATERIALS & METHODS: A U87 cell xenograft model in nude mice was used, with groups: model (M), M + liposomal honokiol (Lip-HNK), M + BEV, and M + Lip-HNK + BEV. Tumor volume, body weight, serum levels of VEGF, VEGFR, TNF-α, and Caspase-3, and expressions of autophagy-related (Beclin-1, LC3) and UPR-related (IRE1, GRP78) molecules in tumor tissues were detected.
Compared with monotherapy, the combination of Lip-HNK and BEV significantly reduced tumor volume and tumor index, decreased serum levels of VEGF, VEGFR, and TNF-α, while increasing serum caspase-3. Further mechanistic studies showed that the combination of Lip-HNK and BEV significantly reduced the expression of Beclin-1, LC3, IRE1, and GRP78 in tumors.
Lip-HNK may promote the anti-GBM effect of BEV by inhibiting autophagy mediated by the UPR response.
研究脂质体厚朴酚是否能增强贝伐单抗（BEV）对胶质母细胞瘤（GBM）的抗肿瘤作用，并探讨其潜在机制。
采用U87细胞裸鼠异种移植模型，分为模型组（M）、M + 脂质体厚朴酚组（Lip-HNK）、M + BEV组和M + Lip-HNK + BEV组。检测肿瘤体积、体重、血清中VEGF、VEGFR、TNF-α和Caspase-3水平，以及肿瘤组织中自噬相关分子（Beclin-1、LC3）和未折叠蛋白反应（UPR）相关分子（IRE1、GRP78）的表达。
与单一疗法相比，Lip-HNK与BEV联合使用显著降低了肿瘤体积和肿瘤指数，降低了血清中VEGF、VEGFR和TNF-α水平，同时提高了血清Caspase-3水平。进一步的机制研究表明，Lip-HNK与BEV联合使用显著降低了肿瘤中Beclin-1、LC3、IRE1和GRP78的表达。
脂质体厚朴酚可能通过抑制UPR反应介导的自噬来促进BEV对GBM的抗肿瘤作用。
