Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, P.R. China.
Anhui Key Laboratory of Brain Function and Diseases, Hefei, Anhui, 230001, P.R. China.
Cell Death Dis. 2023 Jul 17;14(7):443. doi: 10.1038/s41419-023-05959-x.
Glioblastoma (GBM) is the most common malignant primary brain cancer in adults and has constantly been a focus of research. Long noncoding RNAs (lncRNAs) play important roles in the development of cancers. To illustrate the role of lncRNAs in the development of glioblastoma, high-throughput RNA sequencing was performed to obtain the transcripts using three freshly isolated tumor tissue samples from GBM patients and three normal brain tissue samples from the traumatic brain of patients. Then, a lncRNA, MGCG (MGC70870 is expressed at a high level in glioblastoma), which has not been reported previously in GBM, was found to be associated with the prognosis of patients. The results of bioinformatic analysis showed that MGCG was correlated with autophagy and positively correlated with the expression of the autophagy-related gene ATG2A. The data of mass spectrometry demonstrated that the hnRNPK protein was a direct target interacting with MGCG, and MGCG/hnRNPK promoted the development of GBM by enhancing the translation of ATG2A and autophagy. In conclusion, the present study showed that MGCG has the potential to promote the development of GBM and may become a candidate for molecular diagnostics and treatment of tumors.
胶质母细胞瘤（GBM）是成人中最常见的恶性原发性脑癌，一直是研究的重点。长非编码 RNA（lncRNA）在癌症的发展中发挥重要作用。为了说明 lncRNA 在胶质母细胞瘤发展中的作用，使用来自 GBM 患者的三个新鲜分离的肿瘤组织样本和来自创伤性脑患者的三个正常脑组织样本进行了高通量 RNA 测序以获得转录本。然后，发现了一种以前在 GBM 中未报道过的 lncRNA，MGCG（MGC70870 在胶质母细胞瘤中表达水平较高）与患者的预后相关。生物信息学分析的结果表明，MGCG 与自噬相关，并且与自噬相关基因 ATG2A 的表达呈正相关。质谱数据表明，hnRNPK 蛋白是与 MGCG 相互作用的直接靶标，并且 MGCG/hnRNPK 通过增强 ATG2A 和自噬的翻译促进 GBM 的发展。总之，本研究表明 MGCG 具有促进 GBM 发展的潜力，可能成为肿瘤分子诊断和治疗的候选物。
