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白藜芦醇和替莫唑胺通过凋亡信号通路诱导胶质母细胞瘤细胞凋亡并抑制其增殖。

Resveratrol and temozolomide induce apoptosis and suppress proliferation in glioblastoma cells via the apoptotic signaling pathway.

作者信息

Gürsoy Görkem Tutal, Tuncer Mehmet Cudi, Özdemir İlhan

机构信息

Ankara City Hospital - Department of Neurology - Ankara - Turkey.

Dicle University - Faculty of Medicine - Department of Anatomy - Diyarbakir - Turkey.

出版信息

Acta Cir Bras. 2025 Aug 8;40:e405525. doi: 10.1590/acb405525. eCollection 2025.

DOI:10.1590/acb405525
PMID:40802352
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12333572/
Abstract

PURPOSE

Glioblastoma (GBM) is the most common primary brain tumor in the central nervous system. Studies revealing the molecular mechanisms regulating GBM pathogenesis are currently limited. This study aimed to investigate the expression of genes responsible for the apoptotic pathway (p21, p27, p53) after separate and combined application of the natural components resveratrol (Res) and temozolomide (TMZ) in the GBM cell line (U118).

METHODS

In this study, the GBM cell line U118 was used. Apoptotic activation of Res and TMZ via the p21, p27, p53 signaling pathway was evaluated by quantitative reverse transcription polymerase chain reaction and TaLi cytometry. Cell viability was also assessed using the MTT assay.

RESULTS

Res and TMZ inhibited the proliferation and migration of U118 cells. Additionally, Res induced apoptosis by arresting the cell cycle. Moreover, Res treatment upregulated the expression of p27 and p53, which are associated with apoptosis, while it significantly downregulated the expression of the p21 gene.

CONCLUSION

These results indicated that Res and TMZ suppressed the proliferation of GBM cells through apoptotic pathways. Together, Res and TMZ may represent a promising combination for suppressing tumors through apoptotic mechanisms.

摘要

目的

胶质母细胞瘤（GBM）是中枢神经系统中最常见的原发性脑肿瘤。目前揭示调控GBM发病机制分子机制的研究有限。本研究旨在探讨天然成分白藜芦醇（Res）和替莫唑胺（TMZ）单独及联合应用于GBM细胞系（U118）后，凋亡途径相关基因（p21、p27、p53）的表达情况。

方法

本研究使用GBM细胞系U118。通过定量逆转录聚合酶链反应和TaLi细胞计数法评估Res和TMZ通过p21、p27、p53信号通路的凋亡激活情况。还使用MTT法评估细胞活力。

结果

Res和TMZ抑制U118细胞的增殖和迁移。此外，Res通过使细胞周期停滞诱导凋亡。而且，Res处理上调了与凋亡相关的p27和p53的表达，同时显著下调了p21基因的表达。

结论

这些结果表明Res和TMZ通过凋亡途径抑制GBM细胞的增殖。Res和TMZ联合使用可能是通过凋亡机制抑制肿瘤的一种有前景的组合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/5283a6eb79b3/1678-2674-acb-40-e405525-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/9734372a0231/1678-2674-acb-40-e405525-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/994e380cfacf/1678-2674-acb-40-e405525-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/4d4e2250feb7/1678-2674-acb-40-e405525-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/f03afbef07b1/1678-2674-acb-40-e405525-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/cb191b9d83c3/1678-2674-acb-40-e405525-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/86a155c39387/1678-2674-acb-40-e405525-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/1c7887b3d437/1678-2674-acb-40-e405525-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/5283a6eb79b3/1678-2674-acb-40-e405525-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/9734372a0231/1678-2674-acb-40-e405525-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/994e380cfacf/1678-2674-acb-40-e405525-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/4d4e2250feb7/1678-2674-acb-40-e405525-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/f03afbef07b1/1678-2674-acb-40-e405525-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/cb191b9d83c3/1678-2674-acb-40-e405525-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/86a155c39387/1678-2674-acb-40-e405525-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/1c7887b3d437/1678-2674-acb-40-e405525-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b30d/12333572/5283a6eb79b3/1678-2674-acb-40-e405525-gf08.jpg

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Resveratrol ameliorates glioblastoma inflammatory response by reducing NLRP3 inflammasome activation through inhibition of the JAK2/STAT3 pathway.白藜芦醇通过抑制 JAK2/STAT3 通路减少 NLRP3 炎性小体激活，从而改善神经胶质瘤的炎症反应。
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The Combination of 5-FU and Resveratrol Can Suppress the Growth of Glioblastoma Cells Through Downregulation of TRPM2 and β-Catenin.
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Could Metformin and Resveratrol Support Glioblastoma Treatment? A Mechanistic View at the Cellular Level.二甲双胍和白藜芦醇能否支持胶质母细胞瘤的治疗？细胞水平的机制性观点。
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