Coban Ulas, Tekcan Esra, Tural Sengul
Ondokuz Mayıs University, Faculty of Medicine, Department of Obstetrics and Gynecology - Samsun, Turkey.
Ondokuz Mayıs University, Faculty of Medicine, Central Laboratory - Samsun, Turkey.
Rev Assoc Med Bras (1992). 2025 Aug 8;71(7):e20242027. doi: 10.1590/1806-9282.20242027. eCollection 2025.
Recurrent pregnancy loss is characterized as a disorder characterized by two or more miscarriages, and its etiology is unclear. Recent research has focused on microRNA and recurrent pregnancy loss; however, single-nucleotide polymorphisms of microRNA in recurrent pregnancy loss need to be better understood. The aim of the study was to assess if there is any relationship between the miR499b A>G gene polymorphism and recurrent pregnancy loss in Turkish women.
The work has 267 participants, including 153 patients in the recurrent pregnancy loss and 114 participants in the control group. DNA isolation from peripheral blood was conducted using a kit-based approach, followed by the implementation of polymerase chain reaction and restriction fragment length polymorphism methods as outlined in the procedure.
The rates of recurrent pregnancy loss were determined as 32.7% for the AA genotype, 51.6% for the AG genotype, and 15.7% for the GG genotype. When the AA genotype type was taken as a reference, the risk of recurrent pregnancy loss was 0.922 times higher (95%CI 0.513-1.655; p=0.785) in the AG type and 0.354 times lower (95%CI 0.178-0.705; p=0.003) in the GG type. In the recurrent pregnancy loss patient group, the GG genotype was lower than the expected value. The GG genotype was found to be less susceptible to recurrent pregnancy loss development.
Based on our results, GG genotype frequencies, which are the recessive model of the miR499b A>G gene polymorphism, may be a protective genotype in susceptibility to recurrent pregnancy loss in Turkish women. The results obtained from this study represent the first data to be established for the Turkish population.
复发性流产是一种以两次或更多次流产为特征的疾病，其病因尚不清楚。最近的研究集中在微小RNA与复发性流产上；然而，复发性流产中微小RNA的单核苷酸多态性仍需深入了解。本研究的目的是评估土耳其女性中miR499b A>G基因多态性与复发性流产之间是否存在关联。
该研究共有267名参与者，其中包括153名复发性流产患者和114名对照组参与者。采用试剂盒法从外周血中提取DNA，然后按照程序进行聚合酶链反应和限制性片段长度多态性分析。
AA基因型的复发性流产率为32.7%，AG基因型为51.6%，GG基因型为15.7%。以AA基因型为参照，AG基因型复发性流产的风险高0.922倍（95%可信区间0.513 - 1.655；p = 0.785），GG基因型则低0.354倍（95%可信区间0.178 - 0.705；p = 0.003）。在复发性流产患者组中，GG基因型低于预期值。发现GG基因型不易发生复发性流产。
根据我们的研究结果，miR499b A>G基因多态性隐性模型的GG基因型频率，可能是土耳其女性复发性流产易感性中的一种保护基因型。本研究所得结果是针对土耳其人群建立的首批数据。
