对大豆胞囊线虫X12具有抗性的野生大豆的端粒到端粒基因组。

A telomere-to-telomere genome of wild soybean with resistance to soybean cyst nematode X12.

作者信息

Lian Yun, Wu Yongkang, Li Chun, Wei He, Du Pei, Li Jinying, Lei Chenfang, Li Haichao, Wang Shiwei, Zhang Hui, Wang Jinshe, Lu Weiguo

机构信息

Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, China.

出版信息

Sci Data. 2025 Aug 13;12(1):1412. doi: 10.1038/s41597-025-05741-y.

DOI:10.1038/s41597-025-05741-y

PMID:40804058

Abstract

The soybean cyst nematode (SCN) is one of the most destructive pests affecting soybean production worldwide. Wild soybean (Glycine soja) germplasm offers valuable genetic resources for developing SCN-resistant cultivars. Here, we present a telomere-to-telomere assembly of the wild soybean Glycine soja accession YSD56, which is resistant to the highly virulent SCN race X12. The assembled genome has a total length of 1,008.52 Mb, with a contig N50 of 51.97 Mb, and successfully resolves all 20 centromeres and 40 telomeres. The assembly is high quality, with 99.7% completeness based on conserved single-copy orthologs (BUSCO) and a base-level accuracy of QV 52.16. A total of 57,712 protein-coding genes were predicted, 98.79% of which were functionally annotated. This high-quality reference genome provides a valuable resource for investigating SCN resistance and accelerating soybean genetic improvement.

摘要

大豆胞囊线虫（SCN）是影响全球大豆生产的最具破坏性的害虫之一。野生大豆（Glycine soja）种质为培育抗SCN品种提供了宝贵的遗传资源。在此，我们展示了对高毒力SCN小种X12具有抗性的野生大豆Glycine soja种质YSD56的端粒到端粒组装。组装后的基因组全长1008.52 Mb，重叠群N50为51.97 Mb，并成功解析了所有20个着丝粒和40个端粒。该组装质量很高，基于保守单拷贝直系同源基因（BUSCO）的完整性为99.7%，碱基水平准确性为QV 52.16。共预测了57712个蛋白质编码基因，其中98.79%进行了功能注释。这个高质量的参考基因组为研究SCN抗性和加速大豆遗传改良提供了宝贵资源。