埃塞俄比亚中冈德尔地区贡德尔埃尔福拉屠宰场中表面健康的屠宰牛及屠宰场工人感染沙门氏菌的流行情况及抗菌药敏模式

Prevalence and antimicrobial susceptibility patterns of Salmonella from apparently healthy slaughtered cattle and abattoir workers at Gondar Elfora abattoir, Central Gondar Zone, Ethiopia.

作者信息

Tafere Firdyawukal Abuhay, Fenta Melkie Dagnaw, Atanaw Mastewal Birhan, Tsehay Elias Melkamu, Mengstu Yelak Hulugeza, Mebiratu Atsede Solomon

机构信息

Department of Veterinary Pathobiology, College of Veterinary Medicine and Animal Science, University of Gondar, Gondar, Ethiopia.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine and Animal Science, University of Gondar, Gondar, Ethiopia.

出版信息

BMC Microbiol. 2025 Aug 13;25(1):504. doi: 10.1186/s12866-025-04262-3.

DOI:10.1186/s12866-025-04262-3

PMID:40804357

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12345083/

Abstract

BACKGROUND

Salmonella, is among the leading cause of foodborne illnesses in humans, is primarily sourced from food-producing animals. The prevalence of multidrug-resistant Salmonella species has significantly increased in recent years.

METHODS

A cross-sectional study was conducted from November 2022 to May 2023 to determine the prevalence and antimicrobial susceptibility pattern of Salmonella in apparently healthy slaughtered cattle and abattoir personnel at Gondar Elfora abattoir. The study was conducted 253 samples in total, including 75 carcass swabs, 75 liver tissue samples, 75 intestinal contents samples, and 14 stool and 14 water samples from the slaughterhouse. Salmonella isolates were identified using standard isolation and identification techniques. Each isolate was also subjected to Kirby-Bauer disc diffusion tests for antimicrobial susceptibility. STATA version 14 was used to compute and analyses various data from slaughterhouses, antimicrobial susceptibility test results, and animal samples using descriptive statistics.

RESULTS

The overall proportion of Salmonella positive isolates was 13.4%( 253/34) in difference sample sources. The prevalence of Salmonella in cattle was 12% (27/225), of which 17.3% were from carcass swabs, 10.7% were from liver tissue, 8% were from intestinal contents, 14.3% were from human stool, and 35.7% were from water samples. Salmonella isolates were resistant to antimicrobials with specific resistance rates 58.82% for cefoxitin (95%Rn: 42.28 - 75.37), 41.2% (95%Rn: 24.63 - 57.72) for ampicillin and 35.3% (95%Rn: 19.23 - 51.36) for tetracycline. Low resistance pattern was reported in chloramphenicol 17.65% and nalidixic acid 14.71%.

CONCLUSIONS

The study highlights the high prevalence of Antimicrobial-resistant Salmonella, a significant public health concern, emphasizing the need for effective surveillance, control measures, biosecurity, Mandatory abattoir worker training, and Antimicrobial stewardship in livestock.

摘要

背景

沙门氏菌是人类食源性疾病的主要病因之一，主要来源于食用动物。近年来，多重耐药沙门氏菌的流行率显著增加。

方法

于2022年11月至2023年5月进行了一项横断面研究，以确定贡德尔埃尔福拉屠宰场表面健康的屠宰牛和屠宰场工作人员中沙门氏菌的流行率和抗菌药敏模式。该研究共采集了253份样本，包括75份胴体拭子、75份肝脏组织样本、75份肠道内容物样本，以及来自屠宰场的14份粪便和14份水样。使用标准的分离和鉴定技术鉴定沙门氏菌分离株。每个分离株还进行了 Kirby-Bauer 纸片扩散试验以检测抗菌药敏性。使用STATA 14版本通过描述性统计计算和分析来自屠宰场的各种数据、抗菌药敏试验结果和动物样本。

结果

不同样本来源中沙门氏菌阳性分离株的总体比例为13.4%（34/253）。牛中沙门氏菌的流行率为12%（27/225），其中17.3%来自胴体拭子，10.7%来自肝脏组织，8%来自肠道内容物，14.3%来自人类粪便，35.7%来自水样。沙门氏菌分离株对多种抗菌药物耐药，具体耐药率分别为：头孢西丁58.82%（95%Rn：42.28 - 75.37）、氨苄西林41.2%（95%Rn：24.63 - 57.72）、四环素35.3%（95%Rn：19.23 - 51.36）。氯霉素的耐药率为17.65%，萘啶酸的耐药率为14.71%，耐药率较低。