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模式信号分子的生物合成需要太平洋小杆线虫中羧酸酯酶的功能多样化。

Biosynthesis of modular signaling molecules requires functional diversification of carboxylesterases in Pristionchus pacificus.

作者信息

Zhang Pei, Theam Penghieng, Xu Wenya, Ye Shudan, Witte Hanh, Liu Tingwei, Sommer Ralf J, Dong Chuanfu

机构信息

Department of Biology, Faculty of Arts and Sciences, Beijing Normal University, 519087, Zhuhai, China.

The Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Department of Biology, College of Life Sciences, Beijing Normal University, 100875, Beijing, China.

出版信息

Commun Biol. 2025 Aug 13;8(1):1213. doi: 10.1038/s42003-025-08641-4.

DOI:10.1038/s42003-025-08641-4
PMID:40804495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12350714/
Abstract

The entomophilic roundworm Pristionchus pacificus produces a group of complex ascaroside pheromones (e.g., ubas#1), which are built with various intermediates originating from primary metabolic pathways. However, the exact biosynthetic pathways resulting in the production of these modular pheromones remain enigmatic. Here, the application of a workflow combing bioinformatic tools, CRISPR engineering and chemical analysis supports the discovery of three new carboxylesterases Ppa-UAR-5, Ppa-UAR-6 and Ppa-UAR-12 from P. pacificus. Ppa-UAR-5 links two simple ascarosides oscr#9 and ascr#12 to the 2'-position of ubas#3 to furnish the biosynthesis of ubas#1 and ubas#2, whereas Ppa-UAR-12 links two ascr#1 at the 4'-position to synthesize dasc#1. Finally, Ppa-UAR-6 is essential for the biosynthesis of npar#1-3 and part#9. The expression patterns of Ppa-uar-6 and Ppa-uar-12 in intestinal and epidermal cells further suggest pheromone biosynthesis is tissue-specific. These findings support the notion that the expansion and subsequent diversification of carboxylesterases in P. pacificus generates complex modular signaling pheromones.

摘要

嗜虫性蛔虫太平洋前阴线虫产生一组复杂的ascaroside信息素（例如ubas#1），它们由源自初级代谢途径的各种中间体构建而成。然而，导致这些模块化信息素产生的确切生物合成途径仍然不明。在此，结合生物信息学工具、CRISPR基因编辑技术和化学分析的工作流程的应用，支持了从太平洋前阴线虫中发现三种新的羧酸酯酶Ppa-UAR-5、Ppa-UAR-6和Ppa-UAR-12。Ppa-UAR-5将两种简单的ascarosides（oscr#9和ascr#12）连接到ubas#3的2'-位，以完成ubas#1和ubas#2的生物合成，而Ppa-UAR-12在4'-位连接两个ascr#1以合成dasc#1。最后，Ppa-UAR-6对于npar#1-3和part#9的生物合成至关重要。Ppa-uar-6和Ppa-uar-12在肠道和表皮细胞中的表达模式进一步表明信息素生物合成具有组织特异性。这些发现支持了这样一种观点，即太平洋前阴线虫中羧酸酯酶的扩展及其后的多样化产生了复杂的模块化信号信息素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/1ca973d2d011/42003_2025_8641_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/b2b227879a7d/42003_2025_8641_Figa_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/743f659d90ed/42003_2025_8641_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/fdb28c7679fd/42003_2025_8641_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/8e79fe4559e9/42003_2025_8641_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/7ee5454025f2/42003_2025_8641_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/1ca973d2d011/42003_2025_8641_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/b2b227879a7d/42003_2025_8641_Figa_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/743f659d90ed/42003_2025_8641_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/fdb28c7679fd/42003_2025_8641_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/8e79fe4559e9/42003_2025_8641_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/7ee5454025f2/42003_2025_8641_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17ce/12350714/1ca973d2d011/42003_2025_8641_Fig5_HTML.jpg

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本文引用的文献

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