Maksin Sireethon, Kamlangdee Attapon, Boonsoongnern Alongkot, Boonsoongnern Prapassorn
Program of Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
AK Sci and Consulting, Pathum Thani 12000, Thailand.
Animals (Basel). 2025 Jul 24;15(15):2179. doi: 10.3390/ani15152179.
Intestinal alkaline phosphatase (IAP) is a brush border enzyme secreted by enterocytes, playing a crucial role in maintaining gut mucosal defense. This study investigated the expression dynamics of IAP in the small intestine of pigs challenged with K88, compared to healthy controls. Five-week-old pigs (n = 8) were orally administered K88 at a concentration of 2 × 10 CFU/mL, with a dose of 2 mL per pig at 0 and 24 h. Five days post-challenge, tissue samples from the duodenum, jejunum, and ileum were collected for mucosal morphometric analysis and evaluation of IAP expression via immunohistochemistry, Western blotting, and real-time PCR. The results revealed the presence of IAP on the apical surface of villi throughout the small intestine, along with significantly upregulated IAP expression in -challenged pigs compared to controls. These findings suggest that Gram-negative bacteria such as can induce IAP expression, likely through lipopolysaccharide (LPS) stimulation, thereby enhancing its enzymatic activity as part of the intestinal defense mechanism. This study provides insight into the protective role of IAP and highlights its potential as a biomarker for assessing gut health and diagnosing enteric infections in animals.
肠碱性磷酸酶（IAP）是一种由肠上皮细胞分泌的刷状缘酶，在维持肠道黏膜防御中发挥关键作用。本研究调查了与健康对照相比，经K88攻击的猪小肠中IAP的表达动态。5周龄猪（n = 8）口服浓度为2×10 CFU/mL的K88，在0小时和24小时时每头猪剂量为2 mL。攻击后5天，收集十二指肠、空肠和回肠的组织样本用于黏膜形态计量分析，并通过免疫组织化学、蛋白质印迹和实时PCR评估IAP表达。结果显示，在整个小肠绒毛顶端表面均存在IAP，与对照相比，攻击组猪的IAP表达显著上调。这些发现表明，诸如K88这样的革兰氏阴性菌可能通过脂多糖（LPS）刺激诱导IAP表达，从而增强其酶活性，作为肠道防御机制的一部分。本研究深入了解了IAP的保护作用，并突出了其作为评估动物肠道健康和诊断肠道感染生物标志物的潜力。
