Yi Qingyuan, Liu Jiaxin, Zhang Yufeng, Qiao Hanzhen, Chen Fang, Zhang Shihai, Guan Wutai
Guangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, China.
College of Biological Engineering, Henan University of Technology, Zhengzhou, China.
Front Microbiol. 2021 Mar 25;12:647242. doi: 10.3389/fmicb.2021.647242. eCollection 2021.
This study aimed to investigate the effects of dietary anethole supplementation on the growth performance, intestinal barrier function, inflammatory response, and intestinal microbiota of piglets challenged with enterotoxigenic K88. Thirty-six weaned piglets (24 ± 1 days old) were randomly allocated into four treatment groups: (1) sham challenge (CON); (2) K88 challenge (ETEC); (3) K88 challenge + antibiotics (ATB); and (4) K88 challenge + anethole (AN). On day 12, the piglets in the ETEC, ATB, and AN group were challenged with 10 mL K88 (5 × 10 CFU/mL), whereas the piglets in the CON group were orally injected with 10 mL nutrient broth. On day 19, all the piglets were euthanized for sample collection. The results showed that the feed conversion ratio (FCR) was increased in the K88-challenged piglets, which was reversed by the administration of antibiotics or anethole ( < 0.05). The duodenum and jejunum of the piglets in ETEC group exhibited greater villous atrophy and intestinal morphology disruption than those of the piglets in CON, ATB, and AN groups ( < 0.05). Administration of anethole protected intestinal barrier function and upregulated mucosal layer (mRNA expression of mucin-1 in the jejunum) and tight junction proteins (protein abundance of ZO-1 and Claudin-1 in the ileum) of the piglets challenged with K88 ( < 0.05). In addition, administration of antibiotics or anethole numerically reduced the plasma concentrations of IL-1β and TNF-α ( < 0.1) and decreased the mRNA expression of TLR5, TLR9, MyD88, IL-1β, TNF-α, IL-6, and IL-10 in the jejunum of the piglets after challenge with K88 ( < 0.05). Dietary anethole supplementation enriched the abundance of beneficial flora in the intestines of the piglets. In summary, anethole can improve the growth performance of weaned piglets infected by ETEC through attenuating intestinal barrier disruption and intestinal inflammation.
本研究旨在探讨日粮中添加茴香脑对感染产肠毒素K88仔猪生长性能、肠道屏障功能、炎症反应及肠道微生物群的影响。将36头断奶仔猪（24±1日龄）随机分为4个处理组：（1）假攻毒组（CON）；（2）K88攻毒组（ETEC）；（3）K88攻毒+抗生素组（ATB）；（4）K88攻毒+茴香脑组（AN）。在第12天，ETEC、ATB和AN组的仔猪用10 mL K88（5×10⁸CFU/mL）进行攻毒，而CON组的仔猪口服注射10 mL营养肉汤。在第
