An Seong-Won, Kwon Young-Sam
Department of Veterinary Surgery, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
Animals (Basel). 2025 Aug 1;15(15):2260. doi: 10.3390/ani15152260.
Platelet-rich plasma (PRP) is widely applied in veterinary regenerative medicine due to its rich composition of growth factors that promote tissue repair. However, the direct pro-angiogenic function of canine PRP (cPRP) has not been thoroughly validated through controlled in vitro and in vivo experimentation. Human umbilical vein endothelial cells (HUVECs) were used to assess cell proliferation, migration, and tube formation after exposure to cPRP. In addition, a rabbit corneal micropocket assay was employed to evaluate in vivo angiogenic responses. Treatment with 20% cPRP significantly enhanced HUVEC proliferation and migration and induced robust tube formation. In the in vivo model, we observed dose-dependent neovascularization, with the earliest vascular sprouting seen on day 1 in the 40% group. Both models consistently demonstrated that cPRP stimulates vascular development in a concentration-dependent manner. This study provides novel evidence of cPRP's capacity to induce neovascularization, supporting its therapeutic value for treating nonhealing wounds in dogs, especially in cases involving chronic inflammation, aging, or immune dysregulation. These findings offer a scientific foundation for the broader clinical application of cPRP in veterinary regenerative practice.
富含血小板血浆（PRP）因其富含促进组织修复的生长因子而被广泛应用于兽医再生医学。然而，犬PRP（cPRP）直接的促血管生成功能尚未通过可控的体外和体内实验得到充分验证。使用人脐静脉内皮细胞（HUVECs）评估暴露于cPRP后的细胞增殖、迁移和管腔形成。此外，采用兔角膜微袋试验评估体内血管生成反应。用20%的cPRP处理显著增强了HUVEC的增殖和迁移，并诱导了强烈的管腔形成。在体内模型中，我们观察到剂量依赖性的新生血管形成，在40%组中最早在第1天就出现了血管芽生。两个模型均一致表明，cPRP以浓度依赖性方式刺激血管发育。本研究为cPRP诱导新生血管形成的能力提供了新的证据，支持其在治疗犬类难愈合伤口方面的治疗价值，尤其是在涉及慢性炎症、衰老或免疫失调的病例中。这些发现为cPRP在兽医再生实践中的更广泛临床应用提供了科学依据。
