基因转换作为甘蓝型油菜线粒体基因组中插入的叶绿体tRNA基因发生分化的一种机制。
Gene conversion as a mechanism for divergence of a chloroplast tRNA gene inserted in the mitochondrial genome of Brassica oleracea.
作者信息
Dron M, Hartmann C, Rode A, Sevignac M
出版信息
Nucleic Acids Res. 1985 Dec 9;13(23):8603-10. doi: 10.1093/nar/13.23.8603.
Abstract
We have characterized a 1.7 kb sequence, containing a tRNA Leu2 gene shared by the ct and mt genomes of Brassica oleracea. The two sequences are completely homologous except in two short regions where two distinct gene conversion events have occurred between two sets of direct repeats leading to the insertion of 5 bp in the T loop of the mt copy of the ct gene. This is the first evidence that gene conversion represents the initial evolutionary step in inactivation of transferred ct genes in the mt genome. We also indicate that organelle DNA transfer by organelle fusion is an ongoing process which could be useful in genetic engineering.
摘要
我们鉴定了一段1.7 kb的序列，其中包含甘蓝叶绿体和线粒体基因组共有的一个亮氨酸tRNA2基因。这两个序列完全同源，除了在两个短区域，两组同向重复序列之间发生了两个不同的基因转换事件，导致叶绿体基因的线粒体拷贝的T环中插入了5个碱基对。这是基因转换代表线粒体基因组中转移的叶绿体基因失活的初始进化步骤的首个证据。我们还指出，通过细胞器融合进行的细胞器DNA转移是一个持续的过程，这在基因工程中可能有用。
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本文引用的文献
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