Wintz H, Grienenberger J M, Weil J H, Lonsdale D M
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Curr Genet. 1988 Mar;13(3):247-54. doi: 10.1007/BF00387771.
We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of this tRNA(Cys)(GCA) gene with its chloroplast counterpart in wheat suggests that it may be part of a chloroplast DNA insertion into the mitochondrial genome. This gene has been found to be transcribed in the mitochondrion. Two tRNA genes are located on the 5.7 kb BamHI fragment, separated from each other by 250 bp. One is a mitochondrial tRNA(Ser)(GCU) gene. The other, a non-transcribed tRNA(Phe)-like gene, is interrupted by a 49 base-pair inserted DNA sequence in the variable loop and has a Leu (UAA) anticodon.
我们报道了来自玉米线粒体的三个tRNA基因的核苷酸序列。这些基因位于两个BamHI片段中，长度分别为3.55 kb和5.7 kb，与玉米线粒体基因组中的S2序列相邻。在3.55 kb的BamHI片段上，我们鉴定了一个tRNA(Cys)(GCA)基因。该tRNA(Cys)(GCA)基因与其在小麦叶绿体中的对应基因具有很强的序列同源性，这表明它可能是叶绿体DNA插入线粒体基因组的一部分。已发现该基因在线粒体中被转录。两个tRNA基因位于5.7 kb的BamHI片段上，彼此相隔250 bp。一个是线粒体tRNA(Ser)(GCU)基因。另一个是类似非转录tRNA(Phe)的基因，其可变环被一个49个碱基对的插入DNA序列打断，并且具有Leu (UAA)反密码子。
