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mRNA与miRNA分析的整合揭示了油菜（L.）盐胁迫响应的调控机制。

Integration of mRNA and miRNA Analysis Reveals the Regulation of Salt Stress Response in Rapeseed ( L.).

作者信息

Liu Yaqian, Li Danni, Qiao Yutong, Fan Niannian, Gong Ruolin, Zhong Hua, Zhang Yunfei, Lei Linfen, Hu Jihong, Dong Jungang

机构信息

State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling 712100, China.

Population Sciences in the Pacific Program, University of Hawai'i at Mānoa, Honolulu, HI 96813, USA.

出版信息

Plants (Basel). 2025 Aug 4;14(15):2418. doi: 10.3390/plants14152418.

DOI:10.3390/plants14152418
PMID:40805768
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12349528/
Abstract

Soil salinization is a major constraint to global crop productivity, highlighting the need to identify salt tolerance genes and their molecular mechanisms. Here, we integrated mRNA and miRNA profile analyses to investigate the molecular basis of salt tolerance of an elite cultivar S268. Time-course RNA-seq analysis revealed dynamic transcriptional reprogramming under 215 mM NaCl stress, with 212 core genes significantly enriched in organic acid degradation and glyoxylate/dicarboxylate metabolism pathways. Combined with weighted gene co-expression network analysis (WGCNA) and RT-qPCR validation, five candidate genes (, , , , and ) were identified as the regulators of salt tolerance in rapeseed. Haplotype analysis based on association mapping showed that , , and exhibited two major haplotypes that were significantly associated with salt tolerance variation under salt stress in rapeseed. Integrated miRNA-mRNA analysis and RT-qPCR identified three regulatory miRNA-mRNA pairs (bna-miR160a/, novel-miR-126/, and novel-miR-70/) that might be involved in S268 salt tolerance. These results provide novel insights into the post-transcriptional regulation of salt tolerance in , offering potential targets for genetic improvement.

摘要

土壤盐渍化是全球作物生产力的主要限制因素，凸显了鉴定耐盐基因及其分子机制的必要性。在此，我们整合了mRNA和miRNA谱分析，以研究优良品种S268耐盐性的分子基础。时间进程RNA测序分析揭示了在215 mM NaCl胁迫下动态的转录重编程，有212个核心基因在有机酸降解和乙醛酸/二羧酸代谢途径中显著富集。结合加权基因共表达网络分析（WGCNA）和RT-qPCR验证，鉴定出五个候选基因（、、、和）作为油菜耐盐性的调节因子。基于关联作图的单倍型分析表明，、和表现出两种主要单倍型，它们与油菜在盐胁迫下的耐盐性变异显著相关。整合的miRNA-mRNA分析和RT-qPCR鉴定出三个可能参与S268耐盐性的调控miRNA-mRNA对（bna-miR160a/、novel-miR-126/和novel-miR-70/）。这些结果为油菜耐盐性的转录后调控提供了新见解，为遗传改良提供了潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/8496eef2657c/plants-14-02418-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/bcb908b81685/plants-14-02418-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/6ec56e9e01db/plants-14-02418-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/bb741bed8083/plants-14-02418-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/91b75b9a881d/plants-14-02418-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/e1ee6fa4bf74/plants-14-02418-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/3c779b1de2f4/plants-14-02418-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/d0fadfcf1160/plants-14-02418-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/5c2ff414073e/plants-14-02418-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/8496eef2657c/plants-14-02418-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/bcb908b81685/plants-14-02418-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/6ec56e9e01db/plants-14-02418-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/bb741bed8083/plants-14-02418-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/91b75b9a881d/plants-14-02418-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/e1ee6fa4bf74/plants-14-02418-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/3c779b1de2f4/plants-14-02418-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/d0fadfcf1160/plants-14-02418-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/5c2ff414073e/plants-14-02418-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/12349528/8496eef2657c/plants-14-02418-g009.jpg

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