Tian Chunyan, Hua Xiuting, Zhao Peifang, Li Chunjia, Li Xujuan, Liu Hongbo, Liu Xinlong
State Key Laboratory for Tropical Crop Breeding, Kunming 650205, China.
Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661699, China.
Plants (Basel). 2025 Aug 6;14(15):2433. doi: 10.3390/plants14152433.
Fructose-1,6-bisphosphatase (FBP) is a crucial regulatory enzyme in sucrose synthesis and photosynthetic carbon assimilation, functioning through two distinct isoforms: cytosolic FBP (cyFBP) and chloroplastic FBP (cpFBP). However, the identification and functional characterization of genes in remains limited. In this study, we conducted a systematic identification and comparative genomics analyses of in three species. We further examined their expression patterns across leaf developmental zones, spatiotemporal profiles, and responses to diurnal rhythms and hormonal treatments. Our analysis identified 95 genes, including 44 and 51 . Comparative analyses revealed significant divergence in physicochemical properties, gene structures, and motif compositions between the two isoforms. Expression profiling indicated that both and were predominantly expressed in leaves, particularly in maturing and mature zones. During diurnal cycles, their expression peaked around the night-day transition, with exhibiting earlier peaks than . in displayed greater diurnal sensitivity than those in . Hormonal treatments further revealed significant regulatory divergence in genes, both between isoforms and across species. Notably, _ and _ members consistently exhibited higher expression levels across all datasets, suggesting their pivotal roles in sugarcane physiology. These findings not only identify potential target genes for enhancing sucrose accumulation, but also highlight the breeding value of and in sugarcane breeding.
果糖-1,6-二磷酸酶（FBP）是蔗糖合成和光合碳同化过程中的一种关键调节酶，通过两种不同的同工型发挥作用：胞质FBP（cyFBP）和叶绿体FBP（cpFBP）。然而，关于[此处原文缺失相关内容]中基因的鉴定和功能表征仍然有限。在本研究中，我们对三个[此处原文缺失相关内容]物种进行了[此处原文缺失相关内容]的系统鉴定和比较基因组学分析。我们进一步研究了它们在叶片发育区、时空分布以及对昼夜节律和激素处理的响应中的表达模式。我们的分析鉴定出95个[此处原文缺失相关内容]基因，包括44个[此处原文缺失相关内容]和51个[此处原文缺失相关内容]。比较分析揭示了两种同工型在理化性质、基因结构和基序组成上存在显著差异。表达谱分析表明，[此处原文缺失相关内容]和[此处原文缺失相关内容]均主要在叶片中表达，特别是在成熟和成熟区。在昼夜循环中，它们的表达在夜间-白天转换时达到峰值，[此处原文缺失相关内容]的峰值比[此处原文缺失相关内容]更早出现。[此处原文缺失相关内容]中的[此处原文缺失相关内容]比[此处原文缺失相关内容]中的表现出更大的昼夜敏感性。激素处理进一步揭示了[此处原文缺失相关内容]基因在同工型之间和物种之间存在显著的调节差异。值得注意的是，_和_成员在所有数据集中始终表现出较高的表达水平，表明它们在甘蔗生理中的关键作用。这些发现不仅确定了提高蔗糖积累的潜在靶基因，还突出了[此处原文缺失相关内容]和[此处原文缺失相关内容]在甘蔗育种中的育种价值。
