Wu Huafeng, Liu Bin, Cao Yinzhu, Ma Guanpeng, Zheng Xiaowen, Yang Ximeng, Dai Qianli, Zhu Hengxing, Zhu Haoxiang, Song Xingrong, Sui Shunzhao
Chongqing Engineering Research Center for Floriculture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China.
Institute of Horticulture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China.
Plants (Basel). 2025 Aug 7;14(15):2450. doi: 10.3390/plants14152450.
, a traditional ornamental plant in China, is admired for its ability to bloom during the cold winter season and is recognized as an outstanding woody cut flower. MADS-box genes encode transcription factors essential for plant growth and development, with key functions in regulating flowering time and the formation of floral organs. In this study, 74 MADS-box genes () were identified and mapped across 11 chromosomes, with chromosome 1 harboring the highest number (13 genes) and chromosome 3 the fewest (3 genes). Physicochemical property analysis revealed that all CpMADS proteins are hydrophilic and predominantly nuclear-localized. Phylogenetic analysis classified these genes into Type I and Type II subfamilies, highlighting a clear divergence in domain structure. Eighty simple sequence repeat (SSR) loci were detected, with dinucleotide repeats being the most abundant, and the majority located in Type II MADS genes. From 23 samples, 10 polymorphic SSR markers were successfully developed and PCR-validated, enabling a cluster analysis that grouped these cultivars into three distinct clusters. This study offers significant insights into the regulation of flowering, floral organ development, genetic linkage map construction, and the application of marker-assisted selection in .
中国传统观赏植物[植物名称未给出]，因其在寒冷冬季开花的能力而备受赞赏，被视为一种杰出的木本切花。MADS-box基因编码植物生长发育所必需的转录因子，在调节开花时间和花器官形成方面具有关键作用。在本研究中，共鉴定出74个MADS-box基因（[基因相关信息未给出]）并定位到11条染色体上，其中1号染色体上的基因数量最多（13个），3号染色体上的最少（3个）。理化性质分析表明，所有CpMADS蛋白均为亲水性，且主要定位于细胞核。系统发育分析将这些基因分为I型和II型亚家族，突出了结构域结构的明显差异。共检测到80个简单序列重复（SSR）位点，其中二核苷酸重复最为丰富，且大多数位于II型MADS基因中。从23个[样本相关信息未给出]样本中，成功开发并通过PCR验证了10个多态性SSR标记，从而能够进行聚类分析，将这些品种分为三个不同类别。本研究为[植物名称未给出]的开花调控、花器官发育、遗传连锁图谱构建以及标记辅助选择的应用提供了重要见解。
