: Nutrition literacy has garnered growing research attention worldwide, yet only a few instruments have been developed to specifically measure this construct among adolescents. Accordingly, the present research sought to examine the validity and reliability of the Adolescent Nutrition Literacy Scale (ANLS) within a group of Lebanese adolescents. : A cross-sectional study was carried out from December 2022 to March 2023, targeting a nationally representative sample. : Fit indices of the three-factor structure were good. Internal reliability was adequate for the following three subscales: Functional Nutrition Literacy (FNL) (ω = 0.88/α = 0.88), Interactive Nutrition Literacy (INL) (ω = 0.87/α = 0.86) and Critical Nutrition Literacy (CNL) (ω = 0.89/α = 0.89). Invariance was established across genders at configural, metric, and scalar levels. A significantly higher mean FNL and INL scores were found in males compared to females, with no significant difference between the two genders in terms of CNL. Higher FNL, but not CNL and INL scores were significantly associated with lower child food security. : The Arabic ANLS has exhibited robust psychometric reliability, validity, and cost-effectiveness as a tool for assessing nutrition literacy. By utilizing the Arabic version of the ANLS, we can more efficiently and accurately assess the nutritional literacy of adolescents.